Further characterization of distinct high-affinity binding sites for dinotefuran in the abdominal nerve cord of the American cockroach Periplaneta americana (Blattodea),Pesticide Biochemistry and Physiology

当前位置： X-MOL 学术 › Pestic. Biochem. Phys. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Further characterization of distinct high-affinity binding sites for dinotefuran in the abdominal nerve cord of the American cockroach Periplaneta americana (Blattodea)
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.pestbp.2020.104554
Kazuki Nomura ₁ , Ayumi Kawase ₂ , Yoshihisa Ozoe ₃ , Shinichi Banba ₁

Affiliation

Dinotefuran (DTF) is a systemic neonicotinoid insecticide characterized by a tetrahydrofuran ring. In the present study, we examined the characteristics of DTF binding to native nicotinic acetylcholine receptors (nAChRs) expressed in the American cockroach Periplaneta americana using radioligand-binding methods. The Scatchard analysis, using [3H]imidacloprid (IMI), indicated that IMI has a single class of high-affinity binding sites in the P. americana nerve cord. In contrast, the Scatchard analysis using [3H]DTF indicated that DTF has two different classes of binding sites. Both DTF and IMI were found to bind to one of the classes, for which DTF showed low affinity. The other class, for which DTF showed high affinity, was localized in the abdominal nerve cord but not in the thoracic nerve cord. IMI showed low affinity for the high-affinity DTF binding sites. Our data suggest that DTF binds with high affinity to a nAChR subtype distinct from the high-affinity subtype for IMI. This difference might be responsible, at least in part, for the difference in resistance development to DTF and IMI in P. americana.

中文翻译：

美洲蟑螂 Periplaneta americana (Blattodea) 腹部神经索中呋虫胺的独特高亲和力结合位点的进一步表征

呋虫胺 (DTF) 是一种全身性新烟碱类杀虫剂，其特征是四氢呋喃环。在本研究中，我们使用放射性配体结合方法检查了 DTF 与美国大蠊美洲大蠊中表达的天然烟碱乙酰胆碱受体 (nAChR) 结合的特征。使用 [3H] 吡虫啉 (IMI) 的 Scatchard 分析表明，IMI 在美洲假单胞菌神经索中具有单一类别的高亲和力结合位点。相比之下，使用 [3H]DTF 的 Scatchard 分析表明 DTF 具有两种不同类别的结合位点。发现 DTF 和 IMI 都绑定到其中一个类，DTF 显示出低亲和力。另一类 DTF 显示出高亲和力，位于腹神经索中，但不在胸神经索中。IMI 对高亲和力 DTF 结合位点显示出低亲和力。我们的数据表明，DTF 以高亲和力结合与 IMI 高亲和力亚型不同的 nAChR 亚型。这种差异可能至少在一定程度上是导致美洲松中 DTF 和 IMI 抗性发展差异的原因。

更新日期：2020-05-01

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11