Localized and sustained osteogenic-angiogenic stimulation to bone defects is critical for effective bone repair. Here, desferrioxamine (DFO) was loaded on silk fibroin nanofibers and blended with hydroxyapatite nanorods (HA), forming injectable DFO-loaded silk fibroin-HA nanocomposite hydrogels. The composite hydrogels remained homogeneous distribution of HA with high ratio (60 %) and also higher stiffness than that of pure silk fibroin nanofiber hydrogels, which provided stable osteogenic stimulation niches for tissue regeneration. Without the scarify of injectability, the hydrogels achieved slow delivery of DFO for above 60 days, resulting in suitable angiogenesis in vitro and in vivo and better osteogenesis than DFO-free systems. Compared to previous injectable silk fibroin-HA hydrogels, the introduction of vascularization capacity further stimulated the osteogenic differentiation of stem cells and accelerated new bone formation. Quicker and better bone healing were detected at defect sites after the injection of DFO-loaded nanocomposite hydrogels, indicating the effective synergistic effect of osteogenic and angiogenic cues. This work provides a simple and effective strategy of introducing angiogenic cues to bone matrices. We believe that the injectable nanocomposite hydrogels are suitable for the regeneration of bone tissues.

对骨缺损的局部和持续成骨-血管生成刺激对于有效的骨修复至关重要。在这里，去铁胺（DFO）负载在丝素蛋白纳米纤维上，并与羟基磷灰石纳米棒（HA）混合，形成可注射DFO负载的丝素蛋白-HA纳米复合水凝胶。复合水凝胶保持高比例（60％）的HA均匀分布，并且比纯丝素蛋白纳米纤维水凝胶具有更高的刚度，后者为组织再生提供了稳定的成骨刺激壁ni。没有可注射的划破，水凝胶来实现以上60天DFO的缓慢输送，导致合适的血管发生在体外和体内比无DFO的系统具有更好的成骨性。与以前的可注射丝素蛋白-HA水凝胶相比，血管化能力的引入进一步刺激了干细胞的成骨分化并加速了新骨的形成。注射装有DFO的纳米复合水凝胶后，在缺损部位检测到更快，更好的骨愈合，表明成骨和血管生成提示有效的协同作用。这项工作提供了将血管生成提示引入骨基质的简单有效的策略。我们认为可注射的纳米复合水凝胶适合于骨组织的再生。