Abstract In vitro surface coatings are widely used to mimic the role of extracellular matrix in the in vivo environment. Different effects are reported for different surface coatings, however, some of these results are inconsistent across the literature. To explore the role of different surface coatings, we use a new modified stopper-based wound-healing assay, called a stopper assay, with two commonly used surface coatings: gelatin and poly-L-lysine (PLL). Our experimental data shows the gap width decreases faster with the gelatin and PLL coatings. Similarly, the number of cells in certain subregions increases faster with these coatings. Unfortunately, neither of these observations provides definitive mechanistic insight into the role of the coatings. To provide such insight we calibrate the solution of the Fisher-Kolmogorov model to match the experimental data. Our parameter estimates indicate that both coatings significantly increase cell motility without affecting cell proliferation.

中文翻译：

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量化不同表面涂层在伤口愈合实验模型中的作用

摘要 体外表面涂层被广泛用于模拟细胞外基质在体内环境中的作用。不同的表面涂层报告了不同的效果，但是，其中一些结果在文献中不一致。为了探索不同表面涂层的作用，我们使用了一种新的改进的基于塞子的伤口愈合试验，称为塞子试验，具有两种常用的表面涂层：明胶和聚 L-赖氨酸 (PLL)。我们的实验数据表明，使用明胶和 PLL 涂层，间隙宽度减小得更快。同样，使用这些涂层，某些子区域中的细胞数量增加得更快。不幸的是，这些观察结果都没有提供对涂层作用的明确机械洞察力。为了提供这样的洞察力，我们校准了 Fisher-Kolmogorov 模型的解决方案以匹配实验数据。我们的参数估计表明，两种涂层都显着增加了细胞运动性而不影响细胞增殖。