Flash Chromatography System: A Practical Tool for Demonstrating the Influence of Column Characteristics on Chromatographic Resolution,Journal of Chemical Education

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Flash Chromatography System: A Practical Tool for Demonstrating the Influence of Column Characteristics on Chromatographic Resolution
Journal of Chemical Education ( IF 2.5 ) Pub Date : 2020-03-04 , DOI: 10.1021/acs.jchemed.9b00929
Amaury Kasprowiak ₁ , Francine Cazier-Dennin _{1,

2} , Pierre-Edouard Danjou _{1,

2}

Affiliation

A flash purification system using prepacked cartridges containing different silica weights and particle sizes was employed to demonstrate the influence of column characteristics on chromatographic resolution. This chromatographic experiment was a helpful introduction to chromatographic courses where the influence of particle sizes was addressed only theoretically, and it allows students to experience the concept in an active learning style experiment. After running a predefined set of experiments to understand concepts, students were asked to design their own experiment to achieve the best chromatographic resolution. Moreover, the difference of mass versus concentration detectors was highlighted using an ultraviolet (UV) detector and an evaporative light scattering detector (ELSD). Questionnaires were also used to define the students’ initial knowledge and to assess what they learned following this lab.

中文翻译：

快速色谱系统：证明色谱柱特性对色谱分辨率的影响的实用工具

使用包含不同二氧化硅重量和粒径的预装柱的快速纯化系统来证明色谱柱特性对色谱分辨率的影响。该色谱实验是色谱课程的有用介绍，在色谱课程中，仅从理论上解决了粒径的影响，它使学生可以通过积极的学习方式实验来体验这一概念。在运行了一组预定义的实验以理解概念后，要求学生设计自己的实验以实现最佳色谱分辨率。此外，使用紫外线（UV）检测器和蒸发光散射检测器（ELSD）突出显示了质量检测器与浓度检测器的区别。

更新日期：2020-04-24

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