Detection of Circulating Tumor-Related Materials by Aptamer Capturing and Endogenous Enzyme-Signal Amplification.,Analytical Chemistry

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Detection of Circulating Tumor-Related Materials by Aptamer Capturing and Endogenous Enzyme-Signal Amplification.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-03-26 , DOI: 10.1021/acs.analchem.0c00051
Luyao Shen _{1,

2} , Keke Jia ₃ , Tao Bing _{1,

2} , Zhibao Zhang ₁ , Xiaoxiao Zhen ₁ , Xiangjun Liu _{1,

2} , Nan Zhang _{1,

2} , Dihua Shangguan _{1,

2}

Affiliation

Circulating tumor-related materials (CTRMs) shed from original or metastatic tumors, carry a lot of tumor information and are considered as important markers for cancer diagnosis and metastasis prognosis. Herein, we report a colorimetric detection strategy for CTRMs based on aptamer-based magnetic isolation and endogenous alkaline phosphatase (AP)-signal amplification. This strategy exhibited high sensitivity and selectivity toward the CTRMs that express AP heterodimers (the target of aptamer, a potential tumor marker). For clinical samples, this CTRM assay significantly discriminated colorectal cancer patients (n = 50) from healthy individuals (n = 39, p < 0.0001). The receiver operating characteristic (ROC) analysis indicated the sensitivity and specificity reached 92% and 82%, respectively, at the optimal cutoff point, the area under the curve of ROC reached 0.93, suggesting great potential for colorectal cancer diagnosis and therapeutic monitoring. Compared with CTC assays, this strategy is simple and has the potential for point-of-care testing.

中文翻译：

通过适体捕获和内源性酶信号放大检测循环肿瘤相关物质。

循环肿瘤相关物质（CTRM）来源于原始或转移性肿瘤，携带大量肿瘤信息，被认为是癌症诊断和转移预后的重要标志。在这里，我们报告基于适体的磁隔离和内源性碱性磷酸酶（AP）信号放大基于CTRMs的比色检测策略。该策略对表达AP异二聚体（适体的靶标，一种潜在的肿瘤标志物）的CTRM表现出高敏感性和选择性。对于临床样品，该CTRM分析显着区分了大肠癌患者（n = 50）和健康个体（n = 39，p<0.0001）。接受者操作特征（ROC）分析表明，在最佳截止点，ROC曲线下面积达到0.93，灵敏度和特异性分别达到92％和82％，这为大肠癌的诊断和治疗监测提供了巨大的潜力。与CTC分析相比，此策略简单易行，具有即时检验的潜力。

更新日期：2020-03-27

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