The main aim of this study was to investigate the effects of the initial pH values of the buffer on photo-fermentation biohydrogen production. Hydrogen production and the kinetics of it under different initial pH values were analyzed. Effects of initial pH values on reducing sugar consumption, hydrogen production rate, and byproduct production were evaluated at initial pH values of 5-7. The results showed that initial pH values of phosphate buffer had a significant effect on biohydrogen production via photo-fermentation. With the initial pH value of phosphate buffer at 6.0, the cumulative hydrogen production reached its maximum, 569.6 mL. The maximum hydrogen production rate was 23.96 mL/h at the initial pH value of 6.5. With the initial pH values at 5.0 and 7.5, the maximum hydrogen production rates were becoming lower, only 5.59 mL/h and 5.42 mL/h, respectively. And with the increase in pH values, the peak period of hydrogen production was gradually delayed, indicating that the alkaline environment had a negative effect on the ability of photosynthetic bacteria. This study revealed the influence of phosphate buffer initial pH values on the biohydrogen production via photo-fermentation and aimed to provide a scientific reference for further improving the theory and technology for biohydrogen production from biomass.

中文翻译：

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通过磷酸盐缓冲液增强pH值稳定性和光发酵生物制氢。

这项研究的主要目的是研究缓冲液的初始pH值对光发酵生物氢产生的影响。分析了在不同初始pH值下的产氢及其动力学。在初始pH值为5-7时，评估了初始pH值对减少糖消耗，氢产生速率和副产物产生的影响。结果表明，磷酸盐缓冲液的初始pH值对光发酵产生的生物氢产生了显着影响。在磷酸盐缓冲液的初始pH值为6.0的情况下，累积的氢气产生量达到最大值569.6 mL。在初始pH值为6.5时，最大氢气产生速率为23.96 mL / h。在初始pH值为5.0和7.5时，最大氢气产生速率逐渐降低，仅为5.59 mL / h和5。分别为42 mL / h。随着pH值的增加，产氢高峰期逐渐延迟，表明碱性环境对光合细菌的能力产生了负面影响。这项研究揭示了磷酸盐缓冲液初始pH值对通过光发酵产生生物氢的影响，旨在为进一步改进从生物质产生生物氢的理论和技术提供科学参考。