Wastewater plays a major role in water pollution causing transmission of several viral pathogens, including Aichi virus (AiV) and human bocavirus (HBoV), associated with gastrointestinal illness in humans. In this study, we investigated the presence of AiV and HBoV in aquatic, sludge, sediment matrices collected from Abu-Rawash wastewater treatment plant (WWTP), El-Rahawy drain, Rosetta branch of the River Nile in Egypt by conventional polymerase chain reaction (PCR). AiV RNA was detected in 16.6% (2/12), 8.3% (1/12), 8.3% (1/12), 22% (16/72), 12.5% (3/24), 4% (1/24), and 0/24 (0%) of untreated raw sewage, treated sewage, sewage sludge, drainage water, drain sediment, river water, and river sediment, respectively. On the other hand, HBoV DNA was detected in 41.6% (5/12), 25% (3/12), 16.6% (2/12), 48.6% (35/72), 29% (7/24), 3/24 (12.5%), 4% (1/24) of untreated raw sewage, treated raw sewage, sewage sludge, drainage water, drain sediment, river water, and river sediment, respectively. This study provides data on the presence of these viruses in various types of water samples that are valuable to environmental risk assessment. In addition, the current study demonstrates the importance of environmental monitoring as an additional tool to investigate the epidemiology of AiV and HBoV circulating in a given community.

中文翻译：

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埃及废水处理厂，排水沟和尼罗河中样品中的爱知病毒和人类博卡病毒的环境监测。

废水在水污染中起主要作用，导致多种病毒病原体的传播，包括与人类胃肠道疾病相关的爱知病毒（AiV）和人博卡病毒（HBoV）。在这项研究中，我们调查了通过常规聚合酶链反应从埃及的尼罗河Abu-Rawash废水处理厂（WWTP），El-Rahawy排水管，Rosetta分支收集的水，污泥，沉积物基质中AiV和HBoV的存在（ PCR）。检测到AiV RNA的比例为16.6％（2/12），8.3％（1/12），8.3％（1/12），22％（16/72），12.5％（3/24），4％（1 / 24）和0/24（0％）的未经处理的原污水，经处理的污水，污水污泥，排水，排水沉积物，河水和河流沉积物。另一方面，检测到的HBoV DNA分别为41.6％（5/12），25％（3/12），16.6％（2/12），48.6％（35/72），29％（7/24）， 3/24（12.5％），未经处理的原污水，已处理的原污水，污水污泥，排水，排水沉积物，河水和河流沉积物分别占4％（1/24）。这项研究提供了有关在各种类型的水样本中这些病毒的存在的数据，这些数据对于环境风险评估很有价值。此外，本研究表明，环境监测作为调查特定社区中AiV和HBoV流行病学的附加工具的重要性。