The availability of plant phosphorus in the gut chicken can be improved by increasing phosphorus retention using phytase enzyme or a probiotic with phytase activity as an alternative. In this study, the efficacy of a recombinant probiotic, Lactococcus lactis (L. lactis), with a potential of phytase production was evaluated in broiler chickens. To this aim, 360 one-day-old male broiler Cobb 500 were divided into six treatments with six replicates and reared to 42 days of age. The experimental treatments included positive control diet containing adequate phosphorus (PC), negative control diet containing reduced available phosphorus (NC), negative control diet involving recombinant L. lactis (RLL), negative control diet containing both recombinant L. lactis and Lactobacillus salivarius (RLL + LBS), negative control diet including non-recombinant L. lactis (LL) and negative control diet containing Hostazym® . Growth performance, total tract apparent disappearance of phytate-P and nutrient retention, mineral content of the tibia and histomorphology of jejunum were evaluated at the age of 35 days. Based on the results, the phosphorus (P) deficiency in the diet reduced body weight (BW), average daily gain (ADG), length and strength of tibia and increased feed conversion ratio (FCR) compared to PC group. However, the supplementation of Hostazym® or RLL probiotic into the feed improved BW, ADG, FCR, disappearance of Phytate-P and retention of P, length and strength of the tibia in a level similar to PC treatment. Phosphorus content of tibia in the chickens fed P-deficient diets containing RLL was similar to that of the tibia in the control group. Excreta phytate and total P excretion of the chickens decreased when diets contained Hostazym® , RLL and RLL + LBS. In addition, the diet containing RLL + LBS probiotic increased villi height compared with other treatments (p < .05). Further, recombinant L. lactis could represent phytase activity in the gut environment of the chickens and could be an alternative to the commercial phytase.

中文翻译：

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乳酸乳球菌表达的大肠杆菌肌醇六磷酸酶对肉鸡的营养保留，骨骼特征和肠道形态的影响。

可以通过使用植酸酶或具有植酸酶活性的益生菌作为替代品来增加磷的保留，从而提高肠道鸡中植物磷的利用率。在这项研究中，评估了重组益生菌乳酸乳球菌（乳酸乳球菌）具有植酸酶生产潜力的功效。为此，将360只1天大的雄性肉鸡Cobb 500分为6个处理，重复6次，饲养到42天。实验方法包括：含足够磷（PC）的阳性对照饮食，含有效磷减少的阴性对照饮食（NC），涉及重组乳酸乳球菌（RLL）的阴性对照饮食，含有重组乳酸乳杆菌和唾液乳杆菌的阴性对照饮食（ RLL + LBS），阴性对照饮食，包括非重组L。含Hostazym®的乳酸菌（LL）和阴性对照饮食。在35天龄时评估生长性能，植酸磷的总道表观消失和养分保留，胫骨的矿物质含量以及空肠的组织形态。根据结果​​，与PC组相比，饮食中的磷（P）缺乏减少了体重（BW），平均日增重（ADG），胫骨的长度和强度以及饲料转化率（FCR）增加。但是，在饲料中添加Hostazym®或RLL益生菌可改善体重，ADG，FCR，植酸盐-P的消失和磷的保留，胫骨的长度和强度，其水平与PC处理相似。饲喂含磷低磷饮食的鸡的胫骨磷含量与对照组的胫骨磷含量相似。当日粮中含有Hostazym®，RLL和RLL + LBS时，鸡的排泄植酸盐和总P排泄减少。此外，与其他疗法相比，含RLL + LBS益生菌的饮食可增加绒毛高度（p <.05）。此外，重组乳酸乳球菌可以在鸡的肠道环境中代表肌醇六磷酸酶活性，并且可以替代商业肌醇六磷酸酶。