Mycelial morphogenesis and the production of fungal secretory proteins are still largely unknown. A mutant strain of Aspergillus carbonarius UV-10046 produced abundant polygalacturonase (PG) along with partially saturated canthaxanthin (PSC) at low pH conditions. In the present study, the relationship between PG secretion and PSC biosynthesis was studied using carotenogenic inhibitors and SDS-PAGE electrophoresis. Also the correlation between morphogenesis and PG secretion was investigated by analysing through microscopic studies. From the results, it was observed that secretion of PG was positively influenced by the PSC biosynthesis. The results also showed that the mutant with hairy mycelial structure resulted in higher PG activity when compared to the wild type that lacks hyper branching. From the results, it was confirmed that a mutation might have occurred in the isoprenoid pathway that has helped mutant for survival at acidic conditions. Further, an alteration in the morphogenesis and hyper branching development caused over secretion of PG enzyme in the mutant.

中文翻译：

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形态发生和胡萝卜素发生的变化对炭黑曲霉突变体多聚半乳糖醛酸酶分泌的影响

菌丝体形态发生和真菌分泌蛋白的产生在很大程度上仍是未知的。炭黑曲霉 UV-10046 的突变菌株在低 pH 条件下产生丰富的多聚半乳糖醛酸酶 (PG) 和部分饱和的角黄素 (PSC)。在本研究中，使用类胡萝卜素抑制剂和 SDS-PAGE 电泳研究了 PG 分泌与 PSC 生物合成之间的关系。还通过显微镜研究分析了形态发生和PG分泌之间的相关性。从结果可以看出，PSC 的生物合成对 PG 的分泌有积极的影响。结果还表明，与缺乏超分枝的野生型相比，具有毛状菌丝体结构的突变体导致更高的PG活性。从结果来看，已证实类异戊二烯途径中可能发生了突变，该突变有助于突变体在酸性条件下存活。此外，形态发生和超分支发育的改变导致突变体中PG酶的过度分泌。