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Molecular ring toss of circular BAC DNA using micropillar array for single-molecule studies.
Biomicrofluidics ( IF 2.6 ) Pub Date : 2020-02-21 , DOI: 10.1063/1.5142666
Daiki Dohi 1 , Ken Hirano , Kyohei Terao
Affiliation  

This paper reports a method for trapping circular DNA molecules and imaging the dynamics with high spatial resolution using a micropillar-array device. We successfully trapped circular bacterial artificial chromosome DNA molecules at a micropillar-based "ring toss" in the laminar flow of a microchannel under a fluorescence microscope and demonstrated the imaging of their extension by flow and condensation process induced by spermine solution. DNA molecules were visualized in an extended loop conformation, allowing high spatial resolution, and the results showed that the dynamics is induced by the microfluidic control of the surrounding chemical environment. The method is expected to lead to the elucidation of the physical characteristics and the dynamics of circular DNA molecules.

中文翻译:

使用微柱阵列对环状BAC DNA进行分子环折腾,用于单分子研究。

本文报道了一种使用微柱阵列设备捕获环状DNA分子并以高空间分辨率成像动力学的方法。我们成功地将圆形细菌人工染色体DNA分子捕获在荧光显微镜下微通道层流中基于微柱的“环抛”中,并证明了由精胺溶液诱导的流和冷凝过程对其延伸的成像。DNA分子以扩展的环构象可视化,从而具有较高的空间分辨率,结果表明,动力学是由周围化学环境的微流控引起的。该方法有望导致阐明环状DNA分子的物理特性和动力学。
更新日期:2020-02-21
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