Isolation of an antimicrobial-resistant, biofilm-forming, Klebsiella grimontii isolate from a reusable water bottle.,MicrobiologyOpen

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Isolation of an antimicrobial-resistant, biofilm-forming, Klebsiella grimontii isolate from a reusable water bottle.
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-03-03 , DOI: 10.1002/mbo3.1023
Alasdair T M Hubbard ₁ , Enas Newire ₂ , João Botelho _{3,

4} , Jesús Reiné ₅ , Elli Wright ₁ , Emma A Murphy ₁ , William Hutton ₁ , Adam P Roberts ₁

Affiliation

A reusable water bottle was swabbed as part of the citizen science project “Swab and Send,” and a Klebsiella grimontii isolate was recovered on chromogenic agar and designated SS141. Whole‐genome sequencing of SS141 showed it has the potential to be a human pathogen as it contains the biosynthetic gene cluster for the potent cytotoxin, kleboxymycin, and genes for other virulence factors. The genome also contains the antibiotic‐resistant genes, bla_OXY‐6‐4, and a variant of fosA, which is likely to explain the observed resistance to ampicillin, amoxicillin, and fosfomycin. We have also shown that SS141 forms biofilms on both polystyrene and polypropylene surfaces, providing a reasonable explanation for its ability to colonize a reusable water bottle. With the increasing use of reusable water bottles as an alternative to disposables and a strong forecast for growth in this industry over the next decade, this study highlights the need for cleanliness comparable to other reusable culinary items.

中文翻译：

从可重复使用的水瓶中分离出具有抗药性，可形成生物膜的格氏克雷伯菌。

作为公民科学项目“拭子和发送”的一部分，擦拭了一个可重复使用的水瓶，并在发色琼脂上回收了格氏杆菌（Klebsiella grimontii）分离株，并命名为SS141。SS141的全基因组测序显示它有可能成为人类病原体，因为它包含有效的细胞毒素，克雷伯霉素和其他毒性因子基因的生物合成基因簇。该基因组还包含抗药性基因bla _OXY-6-4和fosA变体，这可能可以解释观察到的对氨苄西林，阿莫西林和磷霉素的抗药性。我们还表明，SS141在聚苯乙烯和聚丙烯表面均会形成生物膜，从而为它在可重复使用的水瓶中定殖的能力提供了合理的解释。随着人们越来越多地使用可重复使用的水瓶代替一次性用品，并且对该行业在未来十年中的增长进行了强有力的预测，这项研究强调了对清洁度与其他可重复使用的烹饪产品相当的需求。

更新日期：2020-03-03

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