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A Europe-wide assessment of antibiotic resistance rates in Bacteroides and Parabacteroides isolates from intestinal microbiota of healthy subjects.
Anaerobe ( IF 2.5 ) Pub Date : 2020-02-29 , DOI: 10.1016/j.anaerobe.2020.102182
József Sóki 1 , Ingrid Wybo 2 , Edit Hajdú 3 , Nurver Ulger Toprak 4 , Samo Jeverica 5 , Catalina-Suzana Stingu 6 , Daniel Tierney 7 , John David Perry 7 , Mária Matuz 8 , Edit Urbán 1 , Elisabeth Nagy 1 ,
Affiliation  

Here, we sought to assess the levels of antibiotic resistance among intestinal Bacteroides and Parabacteroides strains collected between 2014 and 2016 in Europe and also attempted to compare resistance levels between clinical and commensal isolates. Bacteroides and Parabacteroides isolates were recovered from faecal samples via the novel Bacteroides Chromogenic Agar (BCA) method. Antibiotic susceptibilities were determined by agar dilution for ten antibiotics. The values obtained were then statistically evaluated. Altogether 202 Bacteroides/Parabacteroides isolates (of which 24, 11.9%, were B. fragilis) were isolated from the faecal specimens of individuals taken from five European countries. The percentage values of isolates resistant to ampicillin, amoxicillin/clavulanate, cefoxitin, imipenem, clindamycin, moxifloxacin, metronidazole, tetracycline, tigecycline and chloramphenicol were 96.6, 4.5, 14.9, 2.0, 47.3, 11.4, 0, 66.2, 1.5 and 0%, respectively. These values are close to those reported in the previous European clinical Bacteroides antibiotic susceptibility survey except for amoxicillin/clavulanate and clindamycin, where the former was lower and the latter was higher in normal microbiota isolates. To account for these latter findings and to assess temporal effects we compared the data specific for Hungary for the same period (2014–2016), and we found differences in the resistance rates for cefoxitin, moxifloxacin and tetracycline.



中文翻译:

欧洲范围内对健康受试者肠道菌群中拟杆菌和副细菌分离物的抗生素耐药率的评估。

在这里,我们试图评估2014年至2016年在欧洲收集的肠杆菌细菌菌株的抗生素耐药性水平,并试图比较临床分离株和普通分离株之间的耐药水平。杆菌Parabacteroides分离物从经由新颖粪便样品中回收杆菌显色琼脂(BCA)方法。通过琼脂稀释确定十种抗生素的抗生素敏感性。然后对获得的值进行统计评估。共有202种细菌/细菌分离物(其中24种(11.9%)为脆弱芽孢杆菌)是从五个欧洲国家/地区的个人粪便标本中分离出来的。对氨苄西林,阿莫西林/克拉维酸盐,头孢西丁,亚胺培南,克林霉素,莫西沙星,甲硝唑,四环素,替加环素和氯霉素具有抗性的菌株的百分比值为96.6、4.5、14.9、2.0、47.3、11.4、0、66.2、1.5和0%分别。这些值接近以前的欧洲临床杀菌剂中报道的值。除阿莫西林/克拉维酸盐和克林霉素(在正常菌群中前者较低而后者较高)外,抗生素敏感性调查。为了解释后面的这些发现并评估时间影响,我们比较了同期(2014-2016年)针对匈牙利的特定数据,我们发现头孢西丁,莫西沙星和四环素的耐药率存在差异。

更新日期:2020-02-29
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