Food intake is a critical parameter in studies of energy balance, obesity, and metabolic disease, but previous methods to measure food intake in model fish species such as zebrafish (Danio rerio) have been labor intensive or indirect. In this study, we describe a simple and rapid method to measure food intake using freshly hatched brine shrimps as food source, followed by rapid collection using filtration of system water and quantification of collected shrimps using a photometric assay to measure extracted shrimp carotenoid content.

中文翻译：

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一种利用丰年虾测量鱼类食物摄入量的简单快速方法。


食物摄入量是能量平衡、肥胖和代谢疾病研究中的一个关键参数，但以前测量斑马鱼（ Danio rerio ）等模型鱼类食物摄入量的方法都是劳动密集型或间接的。在这项研究中，我们描述了一种简单而快速的方法，使用新鲜孵化的丰年虾作为食物源来测量食物摄入量，然后使用系统水过滤进行快速收集，并使用光度测定法对收集的虾进行定量，以测量提取的虾类胡萝卜素含量。