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A Simple and Rapid Method to Measure Food Intake in Fish Using Brine Shrimp.
Zebrafish ( IF 2 ) Pub Date : 2020-06-12 , DOI: 10.1089/zeb.2019.1820
Zahra Mashhadi 1 , Hunter Huff Towle 2 , Claus Schneider 1 , Sean S Davies 1
Affiliation  

Food intake is a critical parameter in studies of energy balance, obesity, and metabolic disease, but previous methods to measure food intake in model fish species such as zebrafish (Danio rerio) have been labor intensive or indirect. In this study, we describe a simple and rapid method to measure food intake using freshly hatched brine shrimps as food source, followed by rapid collection using filtration of system water and quantification of collected shrimps using a photometric assay to measure extracted shrimp carotenoid content.

中文翻译:

一种使用盐水虾测量鱼类食物摄入量的简单而快速的方法。

食物摄入能量平衡,肥胖和代谢性疾病,但以往的方法研究的关键参数测量模型鱼类如斑马鱼(食物摄入斑马鱼)已劳动密集型或间接的。在这项研究中,我们描述了一种简单快速的方法来测量食量,方法是使用刚孵化的盐水虾作为食物来源,然后使用系统水过滤快速收集并使用光度测定法定量收集的虾,以测量提取的虾类胡萝卜素含量。
更新日期:2020-06-12
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