Pig cloning technique is valuable in agriculture, biomedicine, and life sciences. However, the full-term developmental efficiency of cloned pig embryos is only about 1%, which limits pig cloning application. The quality of recipient oocytes greatly affects the developmental competence of cloned pig embryos. Thus, this study investigated the effects of a recipient oocyte source (in vivo matured [IVVM] oocytes vs. slaughter house-derived in vitro matured [IVTM] oocytes), and follicular liquid treatment (slaughter house-derived immature follicle-derived fluid [IFF] vs. in vivo-matured follicle-derived fluid [MFF]) during the in vitro maturation (IVM) of oocytes on the development of the cloned pig embryos. Our results showed that using IVVM oocytes to replace IVTM oocytes as recipient oocytes, and using 10% MFF IVM medium to replace 10% IFF IVM medium could enhance the development of the cloned pig embryos. IFF and MFF contained different levels of oocyte quality-related proteins, resulting in different oocyte quality-related gene expression levels and reactive oxygen species levels between the 10% MFF medium-cultured oocytes and 10% IFF medium-cultured oocytes. This study provided useful information for enhancing the pig cloning efficiency by improving the quality of recipient oocytes.

中文翻译：

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收件人卵母细胞体外成熟过程中的来源和卵泡液处理会影响克隆的猪胚胎的发育。

猪克隆技术在农业，生物医学和生命科学中具有重要价值。但是，克隆的猪胚胎的足月发育效率仅为1％左右，这限制了猪克隆的应用。受体卵母细胞的质量极大地影响克隆的猪胚胎的发育能力。因此，本研究调查了受体卵母细胞来源（体内成熟的[IVVM]卵母细胞与屠宰场衍生的体外成熟[IVTM]卵母细胞）和滤泡液治疗（屠宰场衍生的未成熟卵泡衍生液[ IFF]相对体内-matured卵泡衍生流体[MFF]）时的体外卵母细胞的成熟（IVM）对克隆的猪胚胎发育的影响。我们的结果表明，使用IVVM卵母细胞代替IVTM卵母细胞作为受体卵母细胞，并使用10％MFF IVM培养基替代10％IFF IVM培养基可以增强克隆的猪胚胎的发育。IFF和MFF包含不同水平的卵母细胞质量相关蛋白，从而导致10％MFF培养基培养的卵母细胞和10％IFF培养基培养的卵母细胞的卵母细胞质量相关的基因表达水平和活性氧水平不同。这项研究为通过改善受体卵母细胞的质量来提高猪的克隆效率提供了有用的信息。