Background: Breast reconstruction with fat grafting has an unstable retention rate due to insufficient revascularization. Tissue Engineering Chamber (TEC) model can promote tissue regeneration in the chamber by introducing ligated vessels around the tissue. We introduced ligated vessels with free fat graft to investigate the retention rate and revascularization of grafted fat that in TEC model.Methods: SD rats (n=24) was divided into 3 groups randomly. Group A: Standard TEC model was constructed; Group B: the epigastric vessel bundles were dissected from the fat flap and ligated, fat flap was cut into granules and planted into the chamber; Group C: Free fat was planted in the chamber. At week 6, samples in the chamber were harvested.Results: Significant volume increase was observed in group A and B, while the volume decreased in group C (P<0.05). Regeneration morphology could be found according to the histological observation in A and B. Micro CT results showed the ligated vessels into grafted fat sprouting robustly, coordinated with volume changes.Conclusion: Fat grafts in TEC model could not only survive but also regenerate. The combination of fat graft and TEC could fabricate a vascularized fat flap, which was a promising method in breast reconstruction.Abbreviations: VOI: Volumes of Interest; TEC: Tissue Engineering Chamber; CAL: Cell Assisted Lipotransfer.

中文翻译：

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结扎血管的引入促进游离脂肪的保留和再生：在组织工程室中构建脂肪瓣。

背景：由于血运重建不足，脂肪移植重建乳房的保留率不稳定。组织工程腔室（TEC）模型可以通过在组织周围引入结扎的血管来促进腔室内的组织再生。方法：SD大鼠（n = 24）随机分为3组，每组24只。A组：建立标准TEC模型；B组：将上皮血管束从脂肪皮瓣上切开并结扎，将脂肪皮瓣切成颗粒并植入小室。C组：在室中种植游离脂肪。结果在第6周时，收集了房间中的样品。结果：A和B组的体积明显增加，而C组的体积减小（P <0。05）。根据A，B组织学观察，可以发现其再生形态。显微CT结果显示，结扎的血管牢固地嫁接到嫁接的脂肪中，并与体积变化密切相关。结论：TEC模型中的脂肪移植物不仅可以存活，而且可以再生。脂肪移植物和TEC的组合可以制造血管化的脂肪瓣，这在乳房重建中是一种很有前途的方法。TEC：组织工程室；CAL：细胞辅助脂质转移。脂肪移植物和TEC的组合可以制造血管化的脂肪瓣，这在乳房重建中是一种很有前途的方法。TEC：组织工程室；CAL：细胞辅助脂质转移。脂肪移植物和TEC的组合可以制造血管化的脂肪瓣，这在乳房重建中是一种很有前途的方法。TEC：组织工程室；CAL：细胞辅助脂质转移。