Four undescribed alkaloids, 7-ethoxy-6-methoxy-2-methylisoquinolin-1(2H)-one, 7,8-dihydroxy-6-methoxy-2-methylisoquinolin-1(2H)-one, N-formylhernagine, and 5,6-dihydroxy-N-methylphthalimide, were obtained from the root bark of Hernanadia nymphaeifolia, along with fourteen known compounds. The structures of these compounds were determined through spectroscopic and MS analyses. 7,8-Dihydroxy-6-methoxy-2-methylisoquinolin-1(2H)-one, N-formylhernagine, 5,6-dihydroxy-N-methylphthalimide, oxohernagine, hernandonine, and N-trans-feruloylmethoxytyramine inhibited the superoxide anion (O2-) production (IC50 values ≤ 6.23 μg/mL) by neutrophils stimulated with formyl-L-methionyl-L-leuckyl-L-phenyl-alanine/cytochalasin B (fMLP/CB). Furthermore, 7,8-dihydroxy-6-methoxy-2-methylisoquinolin-1(2H)-one, N-formylhernagine, 5,6-dihydroxy-N-methylphthalimide, oxohernagine, and N-trans-feruloylmethoxytyramine inhibited fMLP/CB-induced elastase release with IC50 values ≤ 7.41 μg/mL. In addition, 7,8-dihydroxy-6-methoxy-2-methylisoquinolin-1(2H)-one, N-formylhernagine, oxohernagine, and N-trans-feruloylmethoxytyramine showed potent inhibition with IC50 values ≤ 28.55 μM, against lipopolysaccharide (LPS)-induced nitric oxide (NO) generation.

中文翻译：

---

来自睡莲根皮的抗炎生物碱

四种未描述的生物碱、7-乙氧基-6-甲氧基-2-甲基异喹啉-1(2H)-one、7,8-二羟基-6-甲氧基-2-甲基异喹啉-1(2H)-one、N-formylhernagine 和 5 ,6-二羟基-N-甲基邻苯二甲酰亚胺与十四种已知化合物一起从睡莲的根皮中获得。这些化合物的结构是通过光谱和 MS 分析确定的。7,8-二羟基-6-甲氧基-2-甲基异喹啉-1(2H)-one、N-formylhernagine、5,6-dihydroxy-N-methylphthalimide、oxohernagine、hernandonine 和 N-trans-feruloylmethoxytyramine 抑制超氧阴离子（用甲酰-L-甲硫氨酰-L-白氨酰-L-苯基-丙氨酸/细胞松弛素B (fMLP/CB) 刺激的中性粒细胞产生O2-)（IC50 值≤ 6.23 μg/mL）。此外，7,8-二羟基-6-甲氧基-2-甲基异喹啉-1(2H)-酮、N-甲酰基hernagine、5,6-二羟基-N-甲基邻苯二甲酰亚胺、oxohernagine 和 N-反式阿魏酰甲氧基酪胺抑制 fMLP/CB 诱导的弹性蛋白酶释放，IC50 值≤ 7.41 μg/mL。此外，7,8-二羟基-6-甲氧基-2-甲基异喹啉-1(2H)-one、N-formylhernagine、oxohernagine 和 N-trans-feruloylmethoxytyramine 显示出有效的抑制作用，IC50 值≤ 28.55 μM，对脂多糖 (LPS) ) 诱导的一氧化氮 (NO) 生成。