Different densities prerelease packing and times of lethargy in the fruit fly parasitoids Diachasmimorpha longicaudata (Ashmead) were evaluated in order to standardize the process of chilled insect technique for this species. Adults were kept at densities of 0.048, 0.072, 0.096, 0.120, and 0.144 parasitoids/cm2 before release in a México tower, where thermal lethargy was induced at a temperature of 2 ± 2°C for 45 min. Samples of parasitoids were collected to evaluate mortality, survival, fecundity, and flight capacity. All densities showed a similar mortality, both for males (ca. >10%) and females (ca. <7). There was no effect of density on survival and flight capacity in both sexes. On the other hand, fecundity increased with density, 1.66 sons/♀/day, similar to the control. We conclude that a density of 30,000 pupae per cage (0.144 parasitoids/cm2) is adequate for the massive prerelease packaging of the parasitoid D. longicaudata. Regarding the thermal lethargy period, 180 min under 2 ± 2°C conditions, considered as time for management, does not affect the survival, fecundity, and flight capacity of adults. The results obtained are of great utility to establish prerelease packaging parameters for D. longicaudata used in the biological control of Tephritidae fruit fly populations.

中文翻译：

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密度和嗜睡持续时间对果蝇（双翅目：蝇科）的寄生前寄生物Diachassmimorpha longicaudata（膜翅目：Braconidae）的影响。

评价了不同密度的实蝇寄生寄生蝇（Diachasmimorpha longicaudata（Ashmead））中的不同释放前堆积和嗜睡次数，以标准化该物种的冷冻昆虫技术过程。在将其释放到墨西哥塔中之前，将成虫的密度保持在0.048、0.072、0.096、0.120和0.144寄生蜂/平方厘米/ cm2的温度下，在2±2°C的温度下诱导嗜睡45分钟。收集了寄生物样品以评估死亡率，存活率，繁殖力和飞行能力。所有密度的男性（大约> 10％）和女性（大约<7）的死亡率都相似。密度对男女的生存和飞行能力没有影响。另一方面，繁殖力随密度的增加而增加，为1.66子/♀/天，与对照组相似。我们得出的结论是每笼密度为30,000 up（0。144个寄生虫/ cm2）足够用于寄生虫D. longicaudata的大规模预释放包装。关于热性嗜睡期，在2±2°C的条件下180分钟（被视为管理时间）不会影响成年人的生存，繁殖力和飞行能力。所获得的结果对于建立用于龙须科果蝇种群生物防治中的长枝杜鹃的预包装参数具有很大的实用性。