The aim of our study was to determine the antimicrobial susceptibility profiles of 267 Gram-negative clinically significant anaerobes, isolated between October 2016 and October 2019, in a Greek university hospital.

The species identification was performed by conventional methods and using the Vitek 2 automated system. Antimicrobial susceptibility testing to determine the MICs was performed by the E-test method. The antimicrobial agents tested were penicillin, ampicillin, amoxicillin-clavulanic acid, piperacillin-tazobactam, cefoxitin, imipenem, meropenem, clindamycin, metronidazole, moxifloxacin, chloramphenicol and tigecycline. The results were interpreted using the CLSI and FDA breakpoints.

The majority of the isolates belonged to Bacteroides fragilis group (58.8%), followed by Prevotella spp. (23.2%), Fusobacterium spp. (11.2%) and Veillonella spp. (6.4%). The most prevalent types of infection were skin and soft tissue infections (34.8%), and inta-abdomonal infections (29.6%). Among all isolates tested, the lowest rates of resistance (<5%) were detected to carbapenems, metronidazole, chloramphenicol and tigecycline. Resistance to piperacillin-tazobactam was observed in 5.4%, 24.6%, 3.3% and 17.6%, of B. fragilis, B. fragilis group, Fusobacterium spp. and Veillonella spp. isolates, respectively. Although a high prevalence of resistance to clindamycin, cefoxitin, and moxifloxacin, was detected particularly among members of the B. fragilis group, cefoxitin resistance was low for Prevotella spp. (3.2%), Fusobacterium spp. (3.3%) and Veillonella spp. (0%).

Our findings underscore the need for periodic monitoring of antimicrobial resistance in order to guide empirical therapy.

我们研究的目的是确定2016年10月至2019年10月之间在希腊大学医院分离的267例革兰氏阴性临床上重要的厌氧菌的抗菌药敏性。

通过常规方法并使用Vitek 2自动化系统进行物种鉴定。通过E-test方法进行确定MIC的抗菌药敏试验。所测试的抗菌剂为青霉素，氨苄西林，阿莫西林-克拉维酸，哌拉西林-他唑巴坦，头孢西丁，亚胺培南，美罗培南，克林霉素，甲硝唑，莫西沙星，氯霉素和替加环素。使用CLSI和FDA断点解释了结果。

多数分离物属于脆弱拟杆菌（58.8％），其次是普氏杆菌。（23.2％），Fusobacterium spp。（11.2％）和Veillonella spp。（6.4％）。最常见的感染类型是皮肤和软组织感染（34.8％）和腹内感染（29.6％）。在所有测试的分离物中，对碳青霉烯，甲硝唑，氯霉素和替加环素的耐药率最低（<5％）。在脆弱的芽孢杆菌，脆弱的芽孢杆菌组，梭状芽孢杆菌属的5.4％，24.6％，3.3％和17.6％中观察到对哌拉西林-他唑巴坦的抗性。和维永内拉spp。分离。尽管特别是在脆弱的芽孢杆菌组的成员中检测到了对克林霉素，头孢西丁和莫西沙星的高耐药性，但对普雷沃氏菌属的头孢西丁耐药性较低。（3.2％），Fusobacterium spp。（3.3％）和Veillonella spp。（0％）。

我们的发现强调需要定期监测抗菌素耐药性以指导经验疗法。