Endometriosis is a chronic inflammatory disease which is associated with aberrant chemokine expression. We have established a three-dimensional (3D) floating collagen gel culture of human endometriotic cyst stromal cells (ECSCs) as an in vitro model of early-stage fibrosis formation in endometriosis. We evaluated the gene expression profiles of 3D-cultured ECSCs using a gene expression microarray. We identified and confirmed with reverse transcription-polymerase chain reaction that mRNA levels of CXCL1, CXCL2, CXCL3, CXCL8, and CCL20 in 3D-cultured ECSCs were significantly higher than in 2D-cultured ECSCs. The protein levels of CXCL1, CXCL2, CXCL8, and CCL20 in the supernatant of 3D-cultured ECSCs were significantly higher than in 2D-cultured ECSCs. It has been suggested that the 3D-culture model of ECSCs is more suitable for in vitro endometriosis research than 2D-culture. This microarray data provides a new platform to identify the candidate genes involved in the pathogenesis of endometriosis which could be masked in conventional 2D-culture.

中文翻译：

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三维培养中卵巢子宫内膜异位基质细胞的趋化因子表达谱。

子宫内膜异位症是一种慢性炎症性疾病，与趋化因子的异常表达有关。我们已经建立了人类子宫内膜异位囊肿基质细胞（ECSCs）的三维（3D）漂浮胶原凝胶培养，作为子宫内膜异位症早期纤维化形成的体外模型。我们使用基因表达微阵列评估了3D培养的ECSC的基因表达谱。我们鉴定并通过逆转录聚合酶链反应证实，在3D培养的ECSC中，CXCL1，CXCL2，CXCL3，CXCL8和CCL20的mRNA水平显着高于2D培养的ECSC。3D培养的ECSC上清液中CXCL1，CXCL2，CXCL8和CCL20的蛋白质水平显着高于2D培养的ECSC。已经提出，ECSC的3D培养模型比2D培养更适合体外子宫内膜异位研究。这种微阵列数据提供了一个新的平台，用于鉴定参与子宫内膜异位症发病机制的候选基因，这些候选基因可以在常规2D培养中被掩盖。