Three undescribed triterpenes and four previously unreported saponins, along with two known ones, were isolated from shells of Xanthoceras sorbifolium (Sapindaceae). Their structures were elucidated by the interpretation of 1D and 2D NMR data. The nitric oxide (NO) assay revealed that 28-O-isobutyryl-21-O-angeloyl-R1-barrigenol and 3-O-β-D-6-O-methylglucuronopyranosyl-21,22-di-O-angeloyl-R1-barrigenol possessed stronger inhibitory effects on LPS-induced NO overproduction (IC50 = 18.5 ± 1.2 and 28.2 ± 1.8 μM, respectively) than the positive drug minocycline (IC50 = 30.1 ± 1.3 μM) in activated BV2 cells. Western blot, RT-qPCR, and docking experiments further validated that the regulation of iNOS and IL-1β expressions was involved in the anti-neuroinflammatory effects of these two compounds.

中文翻译：

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山梨果壳的化学成分

三种未描述的三萜和四种先前未报道的皂苷，以及两种已知的皂苷，是从山梨黄果（无患子科）的壳中分离出来的。通过对 1D 和 2D NMR 数据的解释阐明了它们的结构。一氧化氮 (NO) 分析表明 28-O-isobutyryl-21-O-angeloyl-R1-barrigenol 和 3-O-β-D-6-O-methylglucuronopyranosyl-21,22-di-O-angeloyl-R1在活化的 BV2 细胞中，-barrigenol 对 LPS 诱导的 NO 过量产生（分别为 IC50 = 18.5 ± 1.2 和 28.2 ± 1.8 μM）具有比阳性药物米诺环素（IC50 = 30.1 ± 1.3 μM）更强的抑制作用。Western印迹、RT-qPCR和对接实验进一步验证了iNOS和IL-1β表达的调节参与了这两种化合物的抗神经炎症作用。