Two novel enzyme-linked immunosorbent assays (ELISAs), designed to detect complexes containing DNA, leucocyte calprotectin and S100A12 proteins, were generated for improved specificity and rapid measurement of neutrophil extracellular traps (NETs). The assays were applied on plasma and serum samples from blood donors for establishment of reference values, and from patients with multiple myeloma (MM) or rheumatoid arthritis (RA) in order to examine putatively increased values in the two different inflammatory conditions. Although NETs were hardly detectable in healthy individuals, NET levels were as expected highly and statistically significantly increased in RA patients. The detection of statistically significantly increased NET levels in MM is a novel finding.

中文翻译：

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NETs通过基于钙卫蛋白的新型检测方法在献血者和多发性骨髓瘤或类风湿关节炎患者中进行了分析：一项初步研究。

产生了两种新颖的酶联免疫吸附测定（ELISA），旨在检测包含DNA，白细胞钙卫蛋白和S100A12蛋白的复合物，以提高特异性并快速测量嗜中性粒细胞胞外捕获物（NETs）。将该测定法应用于献血者和多发性骨髓瘤（MM）或类风湿性关节炎（RA）患者的血浆和血清样本，以建立参考值，以检查两种不同炎症条件下推定值的升高。尽管在健康个体中很难检测到NET，但是在RA患者中NET水平却是很高的，并且在统计学上显着增加。MM中NET水平统计上显着增加的检测是一个新发现。