Periodontitis pathogenesis involves activation of host immune responses triggered by microbial dysbiosis. Therefore, controlling periodontal pathogens in-vivo is a main goal of periodontal therapy. New antimicrobials might help to control periodontal infection and improve treatment outcomes at “the dark times” of increasing antibiotic resistance. Here, we determined the biological activity of collinin and isocollinin against 8 bacterial strains. Antimicrobial activity of collinin and isocollinin, chlorhexidine digluconate (CHX) and sodium hypochlorite (NaClO) was evaluated against clinically relevant periodontal bacteria, like Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Fusobacterium nucleatum, Prevotella intermedia, Dialister pneumosintes strains and superinfectants like Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa strains. A broth microdilution test was carried out to determine the minimum inhibitory concentration of collinin and isocollinin against those strains, and bacterial viability was determined by resazurin assay at diverse concentration and exposure times. P. gingivalis was the most susceptible strain to collinin and isocollinin (MIC 2.1 μg/mL and 4.2 μg/mL respectively). Other periodontal pathogens showed MICs <17 μg/mL for collinin and MICs between 20 and 42 μg/mL for isocollinin, whereas CHX and NaClO showed MICs of 62 and 326 μg/mL, respectively. Collinin and isocollinin also exhibited antimicrobial activity against superinfectant bacteria (MIC < 21 and < 42 μg/mL, respectively). Overall, collinin and isocollinin showed a remarkable antibacterial activity against relevant periodontal and superinfective bacteria, especially against P. gingivalis (MIC 2.1 μg/mL and 4.2 μg/mL respectively) and the highly virulent P. aeruginosa (MIC 5.2 and 20.8 μg/mL, respectively).

牙周炎的发病机制涉及由微生物营养不良触发的宿主免疫反应的激活。因此，体内控制牙周病原体是牙周治疗的主要目标。新抗生素可能有助于控制牙周感染并在抗生素耐药性增强的“黑暗时期”改善治疗效果。在这里，我们确定了Collinin和isocollinin对8个细菌菌株的生物活性。针对临床相关牙周细菌，如骨集线放线菌，牙龈卟啉单胞菌，核梭状芽胞杆菌，中间核小肠杆菌，中间型小肠夜蛾，Dialister pneumosintes，对Collinin和isocollinin，氯己定二葡萄糖酸酯（CHX）和次氯酸钠（NaClO）的抗菌活性进行了评估。菌株和类似的superinfectants大肠杆菌，S taphylococcus 黄色葡萄球菌，和P假单胞菌 铜绿假单胞菌菌株。进行肉汤微稀释试验，以确定Collinin和isocollinin对这些菌株的最低抑制浓度，并通过刃天青测定法在不同浓度和暴露时间下测定细菌生存力。牙龈卟啉单胞菌是对Collinin和isocollinin最敏感的菌株（MIC分别为2.1μg/ mL和4.2μg/ mL）。其他牙周病原体对collinin的MIC小于17μg/ mL，对异collinin的MIC在20至42μg/ mL之间，而CHX和NaClO的MIC分别为62μg/ mL和326μg/ mL。Collinin和isocollinin还显示出对超级感染细菌的抗菌活性（分别为MIC <21和<42μg/ mL）。总体而言，collinin和isocollinin对相关的牙周和超感染细菌表现出显着的抗菌活性，尤其是对牙龈卟啉单胞菌（分别为MIC 2.1μg / mL和4.2μg/ mL）和高毒力的铜绿假单胞菌（MIC 5.2和20.8μg/ mL） ， 分别）。