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Seroprevalence of small ruminant lentivirus (SRLV) infection in wild cervids in Poland.
Preventive Veterinary Medicine ( IF 2.2 ) Pub Date : 2020-01-25 , DOI: 10.1016/j.prevetmed.2020.104905
Monika Olech 1 , Zbigniew Osiński 2 , Jacek Kuźmak 1
Affiliation  

Small ruminant lentiviruses (SRLV) are widespread amongst domesticated sheep and goats worldwide. Infection of wild ruminants in close contact with affected domesticated small ruminants has been proposed as an actor in SRLV epidemiology, but studies are limited. The aim of this study was to estimate the apparent (AP) and estimated prevalence (EP) of exposure to SRLV infection in wild ruminants from Poland. Samples originating from 198 free-living cervids comprising 142 European red deer and 56 roe deer were serologically tested using a multi-epitope recombinant antigen ELISA representing subtypes A1, A13, B1, and B2 of SRLV and a commercial ELISA test. The estimated prevalence of SRLV infection was estimated using the Bayesian approach with models that adjusted for the misclassification of animals because of a small population and lack of sampling method, the imperfect performance of the ELISAs and because sera of different species were tested. The calculated estimated prevalence ranged from 5.3 % (95 % CI 0.3, 12.5) to 24.6 % (95 % CI 3.3, 38.5) for the ELISA with multi-epitope antigens while estimated prevalence using the commercial ELISA was 2.5 % (95 % CI 0.2, 6.6). These results may suggest the existence of a new SRLV reservoir in Poland and highlight the importance of surveilling and controlling SRLV infection in domestic and wild ruminants sharing pasture areas.

中文翻译:

小反刍动物慢病毒(SRLV)感染在波兰野生宫颈中的血清阳性率。

小型反刍动物慢病毒(SRLV)在全世界驯养的绵羊和山羊中广泛分布。已提议与受感染的驯养的小反刍动物密切接触感染野生反刍动物,这是SRLV流行病学的一个主要因素,但研究有限。这项研究的目的是评估波兰野生反刍动物暴露于SRLV感染的表观(AP)和估计患病率(EP)。使用代表SRLV的A1,A13,B1和B2亚型的多表位重组抗原ELISA对源自142个欧洲马鹿和56个ro鹿的198个自由活动鹿的样本进行了血清学测试,并进行了商业ELISA测试。SRLV感染的估计流行率是使用贝叶斯方法估计的,该模型针对因人口少和缺乏采样方法,ELISA的不完善性能以及测试了不同物种的血清而对动物的误分类进行了调整。对于使用多表位抗原的ELISA计算得出的估计患病率为5.3%(95%CI 0.3,12.5)到24.6%(95%CI 3.3,38.5),而使用商业ELISA的估计患病率为2.5%(95%CI 0.2 ,6.6)。这些结果可能表明波兰存在一个新的SRLV水库,并强调了在共享牧场的家庭和野生反刍动物中监测和控制SRLV感染的重要性。对于使用多表位抗原的ELISA计算得出的估计患病率为5.3%(95%CI 0.3,12.5)至24.6%(95%CI 3.3,38.5),而使用商业ELISA的估计患病率为2.5%(95%CI 0.2 ,6.6)。这些结果可能表明波兰存在一个新的SRLV水库,并强调了在共享牧场的家庭和野生反刍动物中监测和控制SRLV感染的重要性。对于使用多表位抗原的ELISA计算得出的估计患病率为5.3%(95%CI 0.3,12.5)至24.6%(95%CI 3.3,38.5),而使用商业ELISA的估计患病率为2.5%(95%CI 0.2 ,6.6)。这些结果可能表明波兰存在一个新的SRLV水库,并强调了在共享牧场的家庭和野生反刍动物中监测和控制SRLV感染的重要性。
更新日期:2020-01-25
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