Supplementation of folic acid (FA) is beneficial to several neurological diseases because it promotes notch signaling and neurogenesis and reduces blood homocysteine levels. We hypothesized that postischemic supplementation of FA is beneficial for neuronal survival and regeneration. The objective of the present study was to determine the postischemic neuroprotective and neuroregenerative efficacy of FA supplementation and its effects on various cellular processes in vitro. This work benefited from the use of FA and glucose-free media to better assess the ischemic neuroprotection provided by FA supplementation. The postischemic supplementation of FA significantly improved cell viability, and the improvement was primarily by obstructing the oxygen-glucose deprivation (OGD)-activated apoptosis. Furthermore, postischemic treatment with FA significantly reduced the mitochondrial membrane depolarization and the formation of acidic organelles triggered by OGD. Moreover, FA's effect on neuroregeneration following OGD was evaluated by measuring the cell proliferation and neurite outgrowth length. Treatment with FA enhanced cell proliferation and neurite outgrowth significantly. Thus, these results revealed some of the mechanisms by which FA supplementation provided neuroprotection and neuroregeneration following ischemic injury and highlighted the need for further research into the potential of folic acid as a clinical drug for ischemic stroke.

中文翻译：

---

缺血后补充叶酸可改善体外神经元存活和再生

补充叶酸 (FA) 对多种神经系统疾病有益，因为它可以促进缺口信号和神经发生并降低血液同型半胱氨酸水平。我们假设缺血后补充 FA 有利于神经元存活和再生。本研究的目的是确定 FA 补充剂的缺血后神经保护和神经再生功效及其对体外各种细胞过程的影响。这项工作受益于使用 FA 和无葡萄糖培养基，以更好地评估 FA 补充剂提供的缺血性神经保护作用。缺血后补充 FA 显着提高了细胞活力，这种改善主要是通过阻碍氧-葡萄糖剥夺 (OGD) 激活的细胞凋亡。此外，用 FA 进行缺血后处理显着降低了 OGD 引发的线粒体膜去极化和酸性细胞器的形成。此外，通过测量细胞增殖和神经突生长长度来评估 FA 对 OGD 后神经再生的影响。用 FA 处理显着增强了细胞增殖和神经突生长。因此，这些结果揭示了 FA 补充剂在缺血性损伤后提供神经保护和神经再生的一些机制，并强调需要进一步研究叶酸作为缺血性中风临床药物的潜力。通过测量细胞增殖和神经突长出长度来评估对 OGD 后神经再生的影响。用FA处理显着增强细胞增殖和神经突生长。因此，这些结果揭示了 FA 补充剂在缺血性损伤后提供神经保护和神经再生的一些机制，并强调需要进一步研究叶酸作为缺血性中风临床药物的潜力。通过测量细胞增殖和神经突长出长度来评估对 OGD 后神经再生的影响。用 FA 处理显着增强了细胞增殖和神经突生长。因此，这些结果揭示了 FA 补充剂在缺血性损伤后提供神经保护和神经再生的一些机制，并强调需要进一步研究叶酸作为缺血性中风临床药物的潜力。