Intracellular ATP levels influence cell fates in Dictyostelium discoideum differentiation.,Genes to Cells

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Intracellular ATP levels influence cell fates in Dictyostelium discoideum differentiation.
Genes to Cells ( IF 1.3 ) Pub Date : 2020-03-13 , DOI: 10.1111/gtc.12763
Haruka Hiraoka ₁ , Tadashi Nakano ₂ , Satoshi Kuwana ₃ , Masashi Fukuzawa ₄ , Yasuhiro Hirano ₁ , Masahiro Ueda _{1,

5} , Tokuko Haraguchi _{1,

6} , Yasushi Hiraoka _{1,

6}

Affiliation

Multicellular organisms contain various differentiated cells. Fate determination of these cells remains a fundamental issue. The cellular slime mold Dictyostelium discoideum is a useful model organism for studying differentiation; it proliferates as single cells in nutrient-rich conditions, which aggregate into a multicellular body upon starvation, subsequently differentiating into stalk cells or spores. The fates of these cells can be predicted in the vegetative phase: Cells expressing higher and lower levels of omt12 differentiate into stalk cells and spores, respectively. However, omt12 is merely a marker gene and changes in its expression do not influence the cell fate, and determinant factors remain unknown. In this study, we analyzed cell fate determinants in the stalk-destined and spore-destined cells that were sorted based on omt12 expression. Luciferase assay demonstrated higher levels of intracellular ATP in the stalk-destined cells than in the spore-destined cells. Live-cell observation during development using ATP sensor probes revealed that cells with higher ATP levels differentiated into stalk cells. Furthermore, reducing the ATP level by treating with an inhibitor of ATP production changed the differentiation fates of the stalk-destined cells to spores. These results suggest that intracellular ATP levels influence cell fates in D. discoideum differentiation.

中文翻译：

细胞内ATP水平影响盘基网柄菌分化中的细胞命运。

多细胞生物包含各种分化的细胞。这些细胞的命运确定仍然是一个基本问题。细胞粘液霉菌盘基网柄菌是研究分化的有用模型生物。它在营养丰富的条件下以单细胞增殖，饥饿时聚集成多细胞体，随后分化为茎细胞或孢子。这些细胞的命运可以在营养阶段进行预测：表达高水平和低水平omt12的细胞分别分化为茎细胞和孢子。但是，omt12只是一个标记基因，其表达的变化不会影响细胞命运，决定因素仍然未知。在这个研究中，我们分析了根据omt12表达进行分选的以茎为主和以孢子为主的细胞中的细胞命运决定因素。萤光素酶测定法显示，与以孢子为目的地的细胞相比，在以秸秆为目的地的细胞中细胞内ATP的含量更高。在发育过程中使用ATP传感器探针对活细胞进行观察，发现具有较高ATP水平的细胞分化为茎细胞。此外，通过用ATP产生的抑制剂进行处理来降低ATP的水平，将以茎为目标的细胞向孢子的分化命运改变了。这些结果表明，胞内ATP水平影响迪斯科菌分化中的细胞命运。在发育过程中使用ATP传感器探针对活细胞进行观察，发现具有较高ATP水平的细胞分化为茎细胞。此外，通过用ATP产生的抑制剂进行处理来降低ATP的水平，将以茎为目标的细胞向孢子的分化命运改变了。这些结果表明，胞内ATP水平影响迪斯科菌分化中的细胞命运。在发育过程中使用ATP传感器探针对活细胞进行观察，发现具有较高ATP水平的细胞分化为茎细胞。此外，通过用ATP产生的抑制剂进行处理来降低ATP的水平，将以茎为目标的细胞向孢子的分化命运改变了。这些结果表明，胞内ATP水平影响迪斯科菌分化中的细胞命运。

更新日期：2020-03-13

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