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Photodynamic efficacy of cercosporin in 3D tumor cell cultures
Photochemistry and Photobiology ( IF 2.6 ) Pub Date : 2020-04-23 , DOI: 10.1111/php.13257
Mantas Grigalavicius 1 , Maria Mastrangelopoulou 1 , Delmon Arous 1, 2 , Asta Juzeniene 1 , Mathilde Ménard 3 , Ellen Skarpen 4 , Kristian Berg 1 , Theodossis A Theodossiou 1
Affiliation  

In the present work, we study the photodynamic action of cercosporin (cerco), a naturally occurring photosensitizer, on human cancer multicellular spheroids. U87 spheroids exhibit double the uptake of cerco than T47D and T98G spheroids as shown by flow cytometry on the single cell level. Moreover, cerco is efficiently internalized by cells throughout the spheroid as shown by confocal microscopy, for all three cell lines. Despite their higher cerco uptake, U87 spheroids show the least vulnerability to cerco‐PDT, in contrast to the other two cell lines (T47D and T98G). While 300 μm diameter spheroids consistently shrink and become necrotic after cerco PDT, bigger spheroids (>500 μm) start to regrow following blue‐light PDT and exhibit high viability. Cerco‐PDT was found to be effective on bigger spheroids reaching 1mm in diameter especially under longer exposure to yellow light (~590 nm). In terms of metabolism, T47D and T98G undergo a complete bioenergetic collapse (respiration and glycolysis) as a result of cerco‐PDT. U87 spheroids also experienced a respiratory collapse following cerco‐PDT, but retained half their glycolytic activity.

中文翻译:

尾孢菌素在 3D 肿瘤细胞培养中的光动力功效

在目前的工作中,我们研究了尾孢菌素 (cerco),一种天然存在的光敏剂,对人类癌症多细胞球体的光动力作用。U87 球体对 cerco 的吸收是 T47D 和 T98G 球体的两倍,如单细胞水平的流式细胞术所示。此外,如共聚焦显微镜所示,对于所有三种细胞系,cerco 被整个球体的细胞有效内化。尽管它们的 cerco 吸收率较高,但与其他两种细胞系(T47D 和 T98G)相比,U87 球体对 cerco-PDT 的脆弱性最低。虽然 300 μm 直径的球体在 cerco PDT 后不断缩小并坏死,但更大的球体(> 500 μm)在蓝光 PDT 后开始重新生长并表现出高活力。发现 Cerco-PDT 对直径达到 1 毫米的较大球体有效,尤其是在长时间暴露于黄光(~590 纳米)下。在代谢方面,由于 cerco-PDT,T47D 和 T98G 经历了完全的生物能崩溃(呼吸和糖酵解)。U87 球体在 cerco-PDT 后也经历了呼吸衰竭,但保留了一半的糖酵解活性。
更新日期:2020-04-23
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