Echinocandin B, a kind of antimycotic with cyclic lipo-hexapeptides, was produced by fermentation with Aspergillus nidulans using fructose as main carbon source. The objective of this study was to screen a high-yield mutant capable of using cheap starch as main carbon source by atmospheric and room temperature plasma (ARTP) treatment in order to decrease the production cost of echinocandin B. A stable mutant A. nidulans ZJB19033, which can use starch as optimal carbon source instead of expensive fructose, was selected from two thousands isolates after several cycles of ARTP mutagenesis. To further increase the production of echinocandin B, the optimization of fermentation medium was performed by response surface methodology (RSM), employing Plackett-Burman design (PBD) followed by Box-Behnken design (BBD). The optimized fermentation medium provided the optimal yield of echinocandin B, 2425.9 ± 43.8 mg/L, 1.3-fold compared to unoptimized medium. The results indicated that the mutant could achieve high echinocandin B production using cheap starch as main carbon source, and the cost of carbon sources in fermentation medium reduced dramatically by about 45%.

以果糖为主要碳源，用构巢曲霉发酵生产了一种带有环脂六肽的抗真菌药棘球素B。这项研究的目的是通过大气和室温等离子体（ARTP）处理筛选能够利用廉价淀粉作为主要碳源的高产突变体，以降低棘皮菌素B的生产成本。一种稳定的突变体构巢曲霉ZJB19033可通过使用淀粉作为最佳碳源而不是昂贵的果糖，是经过数次ARTP诱变的2000个分离株中选出的。为了进一步增加棘皮菌素B的产量，发酵表面的优化是通过响应面方法（RSM）进行的，采用Plackett-Burman设计（PBD），然后采用Box-Behnken设计（BBD）。优化的发酵培养基提供了棘球菌素B的最佳产量，为2425.9±43.8 mg / L，是未优化培养基的1.3倍。结果表明，该突变体可利用廉价的淀粉作为主要碳源，实现高棘皮菌素B的生产，发酵培养基中碳源的成本大幅降低了约45％。