The gliomagenesis remains not fully established and their etiological factors still remain obscure. Polyomaviruses were detected and involved in several human tumors. Their potential implication in gliomas has been not yet surveyed in Africa and Arab World. Herein, we investigated the prevalence of six polyomaviruses (SV40, JCPyV, BKPyV, MCPyV, KIPyV, and WUPyV) in 112 gliomas from Tunisian patients. The DNA sequences of polyomaviruses were examined by PCR assays. Viral infection was confirmed by DNA in situ hybridization (ISH) and/or immunohistochemistry (IHC). The relationships between polyomavirus infection and tumor features were evaluated. Specific SV40 Tag, viral regulatory, and VP1 regions were identified in 12 GBM (10.7%). DNA ISH targeting the whole SV40 genome and SV40 Tag IHC confirmed the PCR findings. Five gliomas yielded JCPyV positivity by PCR and DNA ISH (2.7%). However, no BKPyV, KIPyV, and WUPyV DNA sequences were identified in all samples. MCPyV DNA was identified in 30 gliomas (26.8%). For GBM samples, MCPyV was significantly related to patient age (p = 0.037), tumor recurrence (p = 0.024), and SV40 (p = 0.045) infection. No further significant association was identified with the remaining tumor features (p > 0.05) and patient survival (Log Rank, p > 0.05). Our study indicates the presence of SV40, JCPyV, and MCPyV DNA in Tunisian gliomas. Further investigations are required to more elucidate the potential involvement of polyomaviruses in these destructive malignancies.

中文翻译：

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胶质瘤中猿猴病毒40（SV40）和人类JC，BK，MC，KI和WU多瘤病毒的调查。

胶质瘤的发生仍未完全建立，其病因仍不清楚。多瘤病毒被发现并参与了几种人类肿瘤。在非洲和阿拉伯世界尚未调查它们对神经胶质瘤的潜在影响。在这里，我们调查了来自突尼斯患者的112例神经胶质瘤中六种多瘤病毒（SV40，JCPyV，BKPyV，MCPyV，KIPyV和WUPyV）的流行情况。通过PCR测定检查多瘤病毒的DNA序列。DNA原位杂交（ISH）和/或免疫组织化学（IHC）证实了病毒感染。评价多瘤病毒感染与肿瘤特征之间的关系。在12 GBM（10.7％）中鉴定出特定的SV40标签，病毒调节区和VP1区。靶向整个SV40基因组和SV40 Tag IHC的DNA ISH证实了PCR结果。五个神经胶质瘤通过PCR和DNA ISH产生JCPyV阳性（2.7％）。但是，在所有样品中均未鉴定出BKPyV，KIPyV和WUPyV DNA序列。在30例神经胶质瘤中检出MCPyV DNA（26.8％）。对于GBM样本，MCPyV与患者年龄（p = 0.037），肿瘤复发（p = 0.024）和SV40（p = 0.045）感染显着相关。其余肿瘤特征（p> 0.05）和患者生存率（对数秩，p> 0.05）没有进一步的显着相关性。我们的研究表明突尼斯神经胶质瘤中存在SV40，JCPyV和MCPyV DNA。需要进一步研究，以进一步阐明多瘤病毒可能参与这些破坏性恶性肿瘤。对于GBM样本，MCPyV与患者年龄（p = 0.037），肿瘤复发（p = 0.024）和SV40（p = 0.045）感染显着相关。其余肿瘤特征（p> 0.05）和患者生存率（对数秩，p> 0.05）没有进一步的显着相关性。我们的研究表明突尼斯神经胶质瘤中存在SV40，JCPyV和MCPyV DNA。需要进一步研究，以进一步阐明多瘤病毒可能参与这些破坏性恶性肿瘤。对于GBM样本，MCPyV与患者年龄（p = 0.037），肿瘤复发（p = 0.024）和SV40（p = 0.045）感染显着相关。其余肿瘤特征（p> 0.05）和患者生存率（对数秩，p> 0.05）没有进一步的显着相关性。我们的研究表明突尼斯神经胶质瘤中存在SV40，JCPyV和MCPyV DNA。需要进一步研究，以进一步阐明多瘤病毒可能参与这些破坏性恶性肿瘤。和突尼斯神经胶质瘤中的MCPyV DNA。需要进一步研究，以进一步阐明多瘤病毒可能参与这些破坏性恶性肿瘤。和突尼斯神经胶质瘤中的MCPyV DNA。需要进一步研究，以进一步阐明多瘤病毒可能参与这些破坏性恶性肿瘤。