Μeagre (Argyrosomus regius) is a newly introduced species in the aquaculture sector characterized by high growth rates and good nutritional value. However, the optimum conditions such as water temperature for the rearing of this species are still under research. The aim of the present study was to investigate several biochemical responses in the heart, muscle, liver, and intestine of meagre reared under different water temperatures (17, 23, and 26 °C) in comparison to the initial acclimation temperature at 20 °C. To assess its cellular stress response, the molecular markers of Hsp70 and Hsp90 induced levels, p38 MAPK and p44/42 MAPK phosphorylated levels, ubiquitin conjugates, and Bcl-2 levels were determined. Moreover, to investigate its antioxidant defense, the activities of superoxide dismutase, catalase, and glutathione reductase were measured. The obtained results in meagre showed a distinct tissue-specific response regarding the variations observed in the studied parameters under different acclimation temperatures. Specifically, most examined tissues presented a decrease in the levels of the examined markers compared to 20 °C, while in the intestine, an increase of Hsp70 and p38 MAPK levels was observed at 23 and 26 °C. However, data on the final weight and length of the fish lead to the conclusion that high water temperatures such as 26 °C might be the best for the rearing of this species. Therefore, it is assumed that the observed differential molecular responses may act in a cell-protective way at all temperatures, irrespectively of the effect of temperature on growth parameters.

中文翻译：

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水温对微弱（Argyrosomus regius）细胞应激反应的影响。

Μeagre（Argyrosomus regius）是水产养殖领域中的一种新近引进物种，具有高生长速率和良好的营养价值。然而，用于饲养该物种的最佳条件如水温仍在研究中。本研究的目的是研究与20°C的初始适应温度相比，在不同水温（17、23和26°C）下饲养的微薄心脏，肌肉，肝脏和肠道中的几种生化反应。为了评估其细胞应激反应，确定了Hsp70和Hsp90诱导水平，p38 MAPK和p44 / 42 MAPK磷酸化水平，泛素结合物和Bcl-2水平的分子标记。此外，为了研究其抗氧化防御作用，测量了超氧化物歧化酶，过氧化氢酶和谷胱甘肽还原酶的活性。在不同的适应温度下，有关研究参数中观察到的变化，获得的微不足道的结果显示出明显的组织特异性响应。具体而言，与20°C相比，大多数受检组织的受检标志物水平降低，而在肠道中，在23和26°C时观察到Hsp70和p38 MAPK水平升高。但是，有关鱼的最终重量和长度的数据得出的结论是，较高的水温（例如26°C）可能是该鱼类的最佳饲养方式。因此，假定观察到的差异分子反应可以在所有温度下以细胞保护的方式起作用，而与温度对生长参数的影响无关。