当前位置: X-MOL 学术Proteome Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Comparative proteomic analysis of the brain and colon in three rat models of irritable bowel syndrome.
Proteome Science ( IF 2.1 ) Pub Date : 2020-02-24 , DOI: 10.1186/s12953-020-0157-9
Beihua Zhang 1 , Hong Xue 1 , Wei Wang 1 , Ting Chen 1 , Min Su 1 , Nan Kang 2 , Jianqin Yang 3 , Zhaoxiang Bian 4 , Fengyun Wang 1 , Xudong Tang 1
Affiliation  

BACKGROUND Irritable bowel syndrome (IBS) has been gradually recognized as a disorder of the brain-gut interaction, but the molecular changes in the brain and colon that occur in disease development remain poorly understood. We employed proteomic analysis to identify differentially expressed proteins in both the brain and colon of three IBS models. METHODS To explore the relevant protein abundance changes in the brain and colon, isobaric tags for relative and absolute quantitation (iTRAQ), liquid chromatography and tandem mass spectrometry (LC-MS) and Western blotting methods were used in three IBS models, including maternal separation (MS, group B), chronic wrap restraint stress (CWRS, group C) and a combination of MS and CWRS (group D). RESULTS We identified 153, 280, and 239 proteins that were common and differentially expressed in the two tissue types of groups B, C and D, respectively; 43 differentially expressed proteins showed the same expression changes among the three groups, including 25 proteins upregulated in the colon and downregulated in the brain, 7 proteins downregulated in the colon and upregulated in the brain, and 3 proteins upregulated and 8 downregulated in both tissues. Gene ontology analysis showed that the differentially expressed proteins were mainly associated with cellular assembly and organization and cellular function and maintenance. Protein interaction network and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that the differentiated proteins were mainly involved in the protein ubiquitination pathway and mitochondrial dysfunction. CONCLUSIONS Taken together, the data presented represent a comprehensive and quantitative proteomic analysis of the brain and colon in IBS models, providing new evidence of an abnormal brain-gut interaction in IBS. These data may be useful for further investigation of potential targets in the diagnosis and treatment of IBS.

中文翻译:

三种肠易激综合征大鼠模型中大脑和结肠的比较蛋白质组学分析。

背景肠易激综合征(IBS)已逐渐被认为是一种脑-肠相互作用的疾病,但在疾病发展过程中发生的大脑和结肠中的分子变化仍然知之甚少。我们采用蛋白质组学分析来鉴定三种 IBS 模型的大脑和结肠中差异表达的蛋白质。方法 为了探索大脑和结肠中相关的蛋白质丰度变化,在包括母体分离在内的三种 IBS 模型中使用了等压标签相对和绝对定量 (iTRAQ)、液相色谱和串联质谱 (LC-MS) 以及蛋白质印迹法(MS,B 组)、慢性包裹约束应力(CWRS,C 组)以及 MS 和 CWRS 的组合(D 组)。结果 我们确定了 153、280、239种蛋白质分别在B组、C组和D组两种组织类型中常见和差异表达;43个差异表达蛋白在三组中表现出相同的表达变化,其中25个蛋白在结肠中上调和在脑中下调,7个在结肠中下调和在脑中上调,3个蛋白在两个组织中上调和8个在两个组织中下调。基因本体分析表明,差异表达蛋白主要与细胞组装和组织以及细胞功能和维持有关。蛋白质相互作用网络和京都基因与基因组百科全书(KEGG)通路分析表明,分化的蛋白质主要参与蛋白质泛素化通路和线粒体功能障碍。结论 综上所述,所提供的数据代表了 IBS 模型中大脑和结肠的全面和定量蛋白质组学分析,为 IBS 中异常的脑-肠相互作用提供了新证据。这些数据可能有助于进一步研究 IBS 诊断和治疗中的潜在目标。
更新日期:2020-02-24
down
wechat
bug