The performance of distinct serological tests (rK39-ICT, IFAT, DAT-LPC, FC-Simplex IgG1) was assessed and a laboratorial algorithm was proposed for accurate diagnosis of VL. DAT-LPC and FC-Simplex IgG1 showed outstanding accuracy (AUC = 0.93) to identify VL patients. The use of a sequential serological algorithm (rK39-ICT screening followed by DAT-LPC or FC-Simplex IgG1) improved the global accuracy for VL (97.2%) diagnosis. An alternative approach for diagnosis of VL has been also assessed for interchangeable use of serum/whole blood lysate samples in DAT-LPC and FC-Simplex IgG1. Our data showed an outstanding agreement for the results obtained with whole blood lysate samples as compared to serum samples (DAT-LPC =100%; FC-Simplex IgG1 = 99%). Together, these findings provide insights to improve the current overall accuracy of VL diagnosis and present innovative laboratorial tests and alternative samples from use in public health services.

中文翻译：

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使用rK39-ICT，DAT-LPC和FC-Simplex IgG1进行内脏利什曼病血清学诊断的实验室算法。

评估了不同血清学检测（rK39-ICT，IFAT，DAT-LPC，FC-Simplex IgG1）的性能，并提出了用于精确诊断VL的实验室算法。DAT-LPC和FC-Simplex IgG1表现出出色的准确性（AUC = 0.93），可识别VL患者。使用顺序血清学算法（rK39-ICT筛选，然后进行DAT-LPC或FC-Simplex IgG1筛选）提高了VL诊断的整体准确性（97.2％）。还评估了另一种诊断VL的方法，以可互换使用DAT-LPC和FC-Simplex IgG1中的血清/全血裂解液样品。我们的数据显示，与血清样品相比，全血裂解液样品获得的结果具有显着一致性（DAT-LPC = 100％； FC-Simplex IgG1 = 99％）。一起，