A variety of natural compounds have been used to reduce the oxidative stress associated with Alzheimer's disease, and many of these defend cells from oxidative stress-induced neuronal toxicity. In this study, the protective effect of radish (Raphanus raphanistrum) extract was investigated in mice and PC12 cells. In vivo behavioral tests were completed to examine the protective effects of the extract on amyloid beta (Aβ)-peptide_1–42-induced learning and memory impairment in a mouse model. The extract increased spontaneous alternation behaviors and step-through latency in mice. We discovered that administration of the extract reduced lipid peroxidation and Aβ aggregation in a biochemical study of mice brain tissues. Treatment with the extract also increased acetylcholine and catalase activity in the brain. Furthermore, the radish extract attenuated H₂O₂-induced oxidative stress in cells. Through sequential fractionation of the radish extract, the active compound was identified as oleamide. These results suggest that the radish extract could have a protective role against oxidative stress-induced neuronal toxicity, possibly owing to the antioxidative activity of oleamide.

中文翻译：

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萝卜提取物对小鼠和PC12细胞神经毒性的保护作用。

多种天然化合物已被用于减轻与阿尔茨海默氏病相关的氧化应激，其中许多天然化合物可保护细胞免受氧化应激诱导的神经元毒性。在这项研究中，萝卜（Raphanus raphanistrum）提取物在小鼠和PC12细胞中的保护作用进行了研究。已完成体内行为测试，以检查提取物对小鼠模型中β淀粉样蛋白（Aβ ）肽_1–42诱导的学习和记忆障碍的保护作用。提取物增加了小鼠的自发交替行为和逐步潜伏期。我们发现该提取物减少脂质过氧化和A的给药β聚集在小鼠脑组织的生化研究中。用提取物治疗还可以增加大脑中的乙酰胆碱和过氧化氢酶活性。此外，萝卜提取物减弱了H ₂ O ₂诱导的细胞氧化应激。通过萝卜提取物的连续分级分离，将活性化合物鉴定为油酰胺。这些结果表明萝卜提取物可能对氧化应激诱导的神经元毒性具有保护作用，这可能是由于油酰胺的抗氧化活性所致。