Osteoporosis is a skeletal disorder, the pathogenic factors of which include the decreased ability of osteogenesis and enhanced osteolysis. Human-derived mesenchymal stem cells (hMSCs) possess the differential capacity to osteoblasts and chondrocytes, so the application of hMSCs in bone tissue is promising to be effective. A group of RNAs which lack of 5′ and 3′ends called circular RNAs (circRNAs) were discovered. In this study, we described a previously found circular RNA, circ_0076906, to bind miR-1305 and regulate its target gene, Osteoglycin (OGN), thus regulate osteogenic differentiation of hMSCs and alleviate the progression of osteoporosis. Osteogenic differentiation induced in hMSCs; qRT-PCR and western blot to examine the expressions of mRNAs and proteins; Alkaline phosphatase activity and Alizarin red staining to examine bone formation; luciferase report experiments to detect the interaction between molecules; and the nuclear/cytoplasm separation of cells. 1. Circ_0076906 was induced in osteogenic differentiation; 2. Circ_0076906 silencing inhibited osteogenesis-related genes in hMSCs; 3. Circ_0076906 acted as a sponge for miR-1305; 4. MiR-1305 regulated OGN expression; 5. Circ_0076906 induced-osteogenic differentiation depended on miR-1305/ OGN pathway. Circ_0076906 relieved osteoporosis and promoted osteogenic differentiation through the miR-1305/ OGN pathway.

骨质疏松症是一种骨骼疾病，其致病因素包括成骨能力下降和溶骨作用增强。人源间充质干细胞（hMSCs）具有与成骨细胞和软骨细胞不同的能力，因此在骨组织中应用hMSCs有望有效。发现了一组缺少5'和3'末端的RNA，称为环状RNA（circRNA）。在这项研究中，我们描述了一个先前发现的环状RNA，circ_0076906，与miR-1305结合并调节其靶基因骨糖蛋白（OGN），从而调节hMSC的成骨分化并减轻骨质疏松的进展。hMSCs中诱导的成骨分化；qRT-PCR和Western blot检测mRNA和蛋白的表达；碱性磷酸酶活性和茜素红染色以检查骨形成；荧光素酶报告实验以检测分子之间的相互作用；以及细胞的核/细胞质分离。1. Circ_0076906诱导成骨分化；2. Circ_0076906沉默抑制hMSCs中成骨相关基因；3. Circ_0076906充当miR-1305的海绵；4. MiR-1305调节的OGN表达；5. Circ_0076906诱导的成骨细胞分化取决于miR-1305 / OGN途径。Circ_0076906通过miR-1305 / OGN途径缓解了骨质疏松并促进了成骨分化。MiR-1305调节OGN表达；5. Circ_0076906诱导的成骨细胞分化取决于miR-1305 / OGN途径。Circ_0076906通过miR-1305 / OGN途径缓解了骨质疏松并促进了成骨分化。MiR-1305调节OGN表达；5. Circ_0076906诱导的成骨细胞分化取决于miR-1305 / OGN途径。Circ_0076906通过miR-1305 / OGN途径缓解了骨质疏松并促进了成骨分化。