Highly sensitive mutation detection methods enable the application of circulating cell-free DNA for molecular tumor profiling. Recent studies revealed that sequencing artifacts, germline variants, and clonal hematopoiesis confound the interpretation of sequencing results and complicate subsequent treatment decision making and disease monitoring. Parallel sequencing of matched white blood cells promises to overcome these issues and enables appropriate variant calling. Comment on: https://doi.org/10.1002/1878-0261.12617.

中文翻译：

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血浆循环无细胞DNA和匹配的白细胞联合突变测序对治疗反应预测的潜力。

高度灵敏的突变检测方法使循环无细胞DNA可以用于分子肿瘤图谱分析。最近的研究表明，测序假象，种系变体和克隆性造血功能会混淆测序结果的解释，并使后续的治疗决策和疾病监测变得复杂。匹配白细胞的平行测序有望克服这些问题，并实现适当的变异检测。评论：https://doi.org/10.1002/1878-0261.12617