Technological advancement in DNA sequencing read-length has drastically changed the quality and completeness of decoded genomes. The aim of this article is not to describe the different technologies of long-read sequencing, or the widely appreciated power of this technology in genome sequencing, assembly, and gene annotation. Instead, in this article, we provide our opinion that with the exception of genome production, transposable element biology is the most radically altered field as a consequence of the advent of long-read sequencing technology. We review how long-reads have been used to answer key questions in transposable element biology, and how in the future long-reads will help elucidate the function of the repetitive fraction of genomes.

中文翻译：

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通过长阅读实现了可转座元素生物学的当前革命。

DNA测序读取长度的技术进步已大大改变了解码基因组的质量和完整性。本文的目的不是描述长时间读取测序的不同技术，也不是描述该技术在基因组测序，组装和基因注释中的广泛认可的功能。取而代之的是，在本文中，我们提供了我们的观点，即由于基因组的产生，由于长期阅读测序技术的出现，转座因子生物学是最根本改变的领域。我们回顾了如何使用长读本来回答转座因子生物学中的关键问题，以及将来如何将长读本来帮助阐明基因组重复部分的功能。