Functions of beta2-adrenergic receptor in human periodontal ligament cells.,Journal of Cellular Biochemistry

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Functions of beta2-adrenergic receptor in human periodontal ligament cells.
Journal of Cellular Biochemistry ( IF 3.0 ) Pub Date : 2020-03-01 , DOI: 10.1002/jcb.29706
Sayuri Hamano _{1,

2} , Atsushi Tomokiyo ₃ , Daigaku Hasegawa ₃ , Asuka Yuda ₄ , Hideki Sugii ₃ , Shinichiro Yoshida ₃ , Hiromi Mitarai ₄ , Naohisa Wada ₄ , Hidefumi Maeda _{1,

3}

Affiliation

Adrenergic receptors (ARs) are receptors of noradrenalin and adrenalin, of which there are nine different subtypes. In particular, β2 adrenergic receptor (β2‐AR) is known to be related to the restoration and maintenance of homeostasis in bone and cardiac tissues; however, the functional role of signaling through β2‐AR in periodontal ligament (PDL) tissue has not been fully examined. In this report, we investigated that β2‐AR expression in PDL tissues and their features in PDL cells. β2‐AR expressed in rat PDL tissues and human PDL cells (HPDLCs) derived from two different patients (HPDLCs‐2G and ‐3S). Rat PDL tissue with occlusal loading showed high β2‐AR expression, while its expression was downregulated in that without loading. In HPDLCs, β2‐AR expression was increased exposed to stretch loading. The gene expression of PDL‐related molecules was investigated in PDL clone cells (2‐23 cells) overexpressing β2‐AR. Their gene expression and intracellular cyclic adenosine monophosphate (cAMP) levels were also investigated in HPDLCs treated with a specific β2‐AR agonist, fenoterol (FEN). Overexpression of β2‐AR significantly promoted the gene expression of PDL‐related molecules in 2 to 23 cells. FEN led to an upregulation in the expression of PDL‐related molecules and increased intracellular cAMP levels in HPDLCs. In both HPDLCs, inhibition of cAMP signaling by using protein kinase A inhibitor suppressed the FEN‐induced gene expression of α‐smooth muscle actin. Our findings suggest that the occlusal force is important for β2‐AR expression in PDL tissue and β2‐AR is involved in fibroblastic differentiation and collagen synthesis of PDL cells. The signaling through β2‐AR might be important for restoration and homeostasis of PDL tissue.

中文翻译：

人牙周膜细胞中β2-肾上腺素能受体的功能。

肾上腺素受体（AR）是去甲肾上腺素和肾上腺素的受体，有九种不同的亚型。特别是，β2 肾上腺素受体 (β2-AR) 已知与骨和心脏组织稳态的恢复和维持有关；然而，牙周膜 (PDL) 组织中 β2-AR 信号传导的功能作用尚未得到充分研究。在本报告中，我们研究了 PDL 组织中的 β2-AR 表达及其在 PDL 细胞中的特征。β2-AR 在来自两个不同患者（HPDLCs-2G 和 ‐3S）的大鼠 PDL 组织和人 PDL 细胞（HPDLCs）中表达。咬合负重的大鼠 PDL 组织中 β2-AR 表达较高，而无负重的大鼠 PDL 组织中 β2-AR 表达下调。在 HPDLC 中，拉伸载荷下β2-AR表达增加。在过表达 β2-AR 的 PDL 克隆细胞（2-23 细胞）中研究了 PDL 相关分子的基因表达。在用特定的 β2-AR 激动剂非诺特罗 (FEN) 处理的 HPDLC 中也研究了它们的基因表达和细胞内环磷酸腺苷 (cAMP) 水平。β2-AR的过度表达显着促进2至23个细胞中PDL相关分子的基因表达。FEN 导致 HPDLC 中 PDL 相关分子表达上调并增加细胞内 cAMP 水平。在两种 HPDLC 中，使用蛋白激酶 A 抑制剂抑制 cAMP 信号传导可抑制 FEN 诱导的 α-平滑肌肌动蛋白基因表达。我们的研究结果表明，咬合力对于 PDL 组织中 β2-AR 的表达很重要，并且 β2-AR 参与 PDL 细胞的成纤维细胞分化和胶原蛋白合成。通过 β2-AR 的信号传导可能对于 PDL 组织的恢复和稳态很重要。

更新日期：2020-03-01

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