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Replication Fork Barriers and Topological Barriers: Progression of DNA Replication Relies on DNA Topology Ahead of Forks.
BioEssays ( IF 3.2 ) Pub Date : 2020-03-01 , DOI: 10.1002/bies.201900204 Jorge B Schvartzman 1 , Pablo Hernández 1 , Dora B Krimer 1
BioEssays ( IF 3.2 ) Pub Date : 2020-03-01 , DOI: 10.1002/bies.201900204 Jorge B Schvartzman 1 , Pablo Hernández 1 , Dora B Krimer 1
Affiliation
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During replication, the topology of DNA changes continuously in response to well-known activities of DNA helicases, polymerases, and topoisomerases. However, replisomes do not always progress at a constant speed and can slow-down and even stall at precise sites. The way these changes in the rate of replisome progression affect DNA topology is not yet well understood. The interplay of DNA topology and replication in several cases where progression of replication forks reacts differently to changes in DNA topology ahead is discussed here. It is proposed, there are at least two types of replication fork barriers: those that behave also as topological barriers and those that do not. Two-Dimensional (2D) agarose gel electrophoresis is the method of choice to distinguish between these two different types of replication fork barriers.
中文翻译：
复制叉障碍和拓扑障碍：DNA 复制的进展依赖于叉前的 DNA 拓扑。
在复制过程中，DNA 的拓扑结构随着众所周知的 DNA 解旋酶、聚合酶和拓扑异构酶的活动而不断变化。然而，replisomes 并不总是以恒定的速度前进，并且会在精确的位置减慢甚至停滞。复制体进展速率的这些变化影响 DNA 拓扑的方式尚不清楚。此处讨论了在复制叉的进展对前面 DNA 拓扑变化的反应不同的几种情况下 DNA 拓扑和复制的相互作用。有人提议，至少有两种类型的复制叉屏障：那些也表现得像拓扑屏障的和那些不表现的。二维 (2D) 琼脂糖凝胶电泳是区分这两种不同类型的复制叉屏障的首选方法。
更新日期：2020-03-01
中文翻译：
复制叉障碍和拓扑障碍：DNA 复制的进展依赖于叉前的 DNA 拓扑。
在复制过程中，DNA 的拓扑结构随着众所周知的 DNA 解旋酶、聚合酶和拓扑异构酶的活动而不断变化。然而，replisomes 并不总是以恒定的速度前进，并且会在精确的位置减慢甚至停滞。复制体进展速率的这些变化影响 DNA 拓扑的方式尚不清楚。此处讨论了在复制叉的进展对前面 DNA 拓扑变化的反应不同的几种情况下 DNA 拓扑和复制的相互作用。有人提议，至少有两种类型的复制叉屏障：那些也表现得像拓扑屏障的和那些不表现的。二维 (2D) 琼脂糖凝胶电泳是区分这两种不同类型的复制叉屏障的首选方法。
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