Excessive use of pesticides in agricultural fields is a matter of great concern for living beings as well as the environment across the world, in particular, the third world countries. Therefore, there is an urgent need to find out an effective way to degrade these hazardous chemicals from the soil in an environment‐friendly way. In the current project, a bacterial species were isolated through enrichment culture from carbofuran‐supplemented rice‐field soil and identified as a carbofuran degrader. The rate of carbofuran degradation by this bacterial species was evaluated using reverse‐phase high‐performance liquid chromatography (RP‐HPLC), which confirmed the ability to utilize as a carbon source up to 4 µg/ml of 99% technical grade carbofuran. The morphological, physiological, biochemical characteristics and phylogenetic analysis of the 16S rRNA sequence showed that this strain belongs to the genus of Enterobacter sp. (sequence accession number LC368285 in DDBJ), and the optimum growth condition for the isolated strain was 37°C at pH 7.0. Moreover, an antibiotic sensitivity test showed that it was susceptible to azithromycin, penicillin, ceftazidime, ciprofloxacin, and gentamycin, and the minimal inhibitory concentration value of gentamycin was 400 μg/ml against the bacteria. It shows beyond doubt from the RP‐HPLC quantification that the isolated bacterium has the ability to detoxify carbofuran (99% pure). Finally, the obtained results imply that the isolated strain of Enterobacter can be used as a potential and effective carbofuran degrader for bioremediation of contaminated sites through bioaugmentation.

中文翻译：

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土传肠杆菌属的功效。克百威降解：降解率的 HPLC 定量

农田农药的过量使用，是全世界，特别是第三世界国家的生物和环境都非常关心的问题。因此，迫切需要找到一种以环境友好的方式从土壤中降解这些有害化学物质的有效方法。在目前的项目中，通过富集培养从添加了呋喃丹的稻田土壤中分离出一种细菌，并鉴定为一种呋喃丹降解剂。使用反相高效液相色谱法 (RP-HPLC) 评估了这种细菌物种对克百威的降解率，这证实了将高达 4 µg/ml 的 99% 工业级克百威用作碳源的能力。形态、生理、16S rRNA 序列的生化特征和系统发育分析表明该菌株属于肠杆菌属。（DDBJ 中的序列登录号 LC368285），分离菌株的最佳生长条件为 37°C，pH 7.0。此外，抗生素敏感性试验表明，其对阿奇霉素、青霉素、头孢他啶、环丙沙星和庆大霉素敏感，庆大霉素的最低抑菌浓度值为400 μg/ml。RP-HPLC 定量表明，分离的细菌具有解毒克百威（纯度 99%）的能力。最后，所得结果表明分离的肠杆菌菌株可作为潜在且有效的克百威降解剂，通过生物强化对污染场地进行生物修复。