Time-resolved temperature-jump infrared absorption spectroscopy at a 0.5 to 1 kHz repetition rate is presented. A 1 kHz neodymium-doped yttrium aluminum garnet (Nd:YAG) laser pumping an optical parametric oscillator provided >70 µJ, 3.75 µm pump pulses, which delivered a temperature jump via excitation of the O–D stretch of a D2O solution. A 10 kHz train of mid-infrared probe pulses was used to monitor spectral changes following the temperature jump. Calibration with trifluoroacetic acid solution showed that a temperature jump of 10 K lasting for tens of microseconds was achieved, sufficient to observe fast processes in functionally relevant biomolecular mechanisms. Modeling of heating profiles across ≤10 µm path length cells and subsequent cooling dynamics are used to describe the initial <100 ns cooling at the window surface and subsequent, >10 µs cooling dynamics of the bulk solution.

中文翻译：

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EXPRESS：高重复率的时间分辨温跃红外光谱

介绍了重复频率为 0.5 到 1 kHz 的时间分辨温度跃变红外吸收光谱。1 kHz 掺钕钇铝石榴石 (Nd:YAG) 激光器泵浦光学参量振荡器提供 >70 µJ、3.75 µm 泵浦脉冲，通过激发 D2O 溶液的 O-D 拉伸提供温度跳跃。使用 10 kHz 中红外探测脉冲序列来监测温度跳跃后的光谱变化。用三氟乙酸溶液校准表明，实现了持续数十微秒的 10 K 温度跳跃，足以观察功能相关生物分子机制中的快速过程。≤10 µm 光程单元的加热曲线建模和随后的冷却动力学用于描述窗口表面和随后的初始 <100 ns 冷却，