A Gram-staining-positive and aerobic coccus with the ability to degrade petroleum bacterium, designated Y42T, was isolated from the Lenghu oil field located in the northern margin of the Qaidam Basin. Phylogenetic and signature nucleotides analyses revealed that strain Y42T belongs to the genus Planococcus. The multiple sequence alignments of 16S rRNA and housekeeping genes showed that strain Y42T formed a distinct lineage with the other Planococcus clade. The average nucleotide identity (ANI) and DNA-DNA hybridization values (DDH) between strain Y42T and the reference strains were 69.5-70.1 and 19.4-21.7%, respectively, which values were below the threshold for species delineation. The major fatty acids of strain Y42T were anteiso-C15:0. The respiratory quinone was MK-7 (71.8%) as the predominant menaquinone followed the MK-6 (28.2%) and the cell-wall hydrolysates contained LL-diaminopimelic acid. The polar lipid was composed of diphosphatidyl glycerol, phosphatidyl glycerol, phosphoglycolipid, aminophospholipid and four unidentified lipids. The peptidoglycan type was A4α (L-Lys-D-Glu). The strain Y42T possessed larger genome (approximately 4 MB) and revealed obvious differences for the abundance of the COG categories compared with the other Planococcus bacteria. Also, the strain Y42T also possessed more unique orthologous proteins. The structural characteristics of the strain Y42T genome provided a competitive advantage for better survival in petroleum-polluted environments. Combined with the 16S rRNA gene and genome sequence, phenotypic as well as chemotaxonomic characterisations, strain Y42T is considered to represent a novel species of the genus Planococcus, for which the name Planococcus lenghuensis sp. nov. be proposed. The type strain is Y42T (= CGMCC 1.15921T = JCM 32719T).

中文翻译：

---

冷湖平球菌 nov。，一种从石油污染的土壤中分离出来的降解石油的细菌。

从位于柴达木盆地北缘的冷湖油田分离出一种具有降解石油细菌能力的革兰氏阳性和好氧球菌，命名为Y42T。系统发育和特征核苷酸分析表明，菌株Y42T属于Planococcus属。16S rRNA和管家基因的多重序列比对表明，菌株Y42T与其他Planococcus进化枝形成了独特的谱系。Y42T菌株和参考菌株之间的平均核苷酸同一性（ANI）和DNA-DNA杂交值（DDH）分别为69.5-70.1和19.4-21.7％，这低于物种划分的阈值。Y42T菌株的主要脂肪酸为anteiso-C15：0。呼吸醌为MK-7（71.8％），主要的甲萘醌紧随MK-6（28）。2％），并且细胞壁水解产物包含LL-二氨基庚二酸。极性脂质由二磷脂酰甘油，磷脂酰甘油，磷酸糖脂，氨基磷脂和四种未鉴定的脂组成。肽聚糖类型为A4α（L-Lys-D-Glu）。Y42T菌株具有更大的基因组（约4 MB），并且与其他Planococcus细菌相比，其COG种类的丰富程度存在明显差异。而且，菌株Y42T还具有更多独特的直系同源蛋白。Y42T菌株基因组的结构特征为在石油污染的环境中更好的生存提供了竞争优势。结合16S rRNA基因和基因组序列，表型以及化学分类学特征，菌株Y42T被认为代表了新的Planococcus菌，其名称为Planhuoccuscus lenghuensis sp。十一月 被提议。类型株是Y42T（= CGMCC 1.15921T = JCM 32719T）。