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TRPA1 modulates noxious odor responses in Lygus hesperus.
Journal of Insect Physiology ( IF 2.3 ) Pub Date : 2020-02-27 , DOI: 10.1016/j.jinsphys.2020.104038
J Joe Hull 1 , Yu-Wen Yang 1 , Katelyn Miyasaki 1 , Colin S Brent 1
Affiliation  

Lygus hesperus isa key pest of many economically important crops across western North America. Central to many aspects of the lives of these insects is chemical signalling, with identified roles in host plant selection, aggregation and passive mate guarding. The development of novel monitoring and control approaches for this insect will rely on a sound understanding of how these cues are perceived and processed, and their impact on behavior. Towards this end, we investigated allyl isothiocyanate, cinnamaldehyde and citronellal, compounds that are noxious repellents to other insects. We found that L. hesperus avoided areas containing the three compounds and that exposure induced increases in movement velocity and duration in both nymphs and adults. This suggests these compounds may work as repellents. To better understand the underlying physiology of this response, RNA interference by dsRNA injection was used to inhibit the expression of two chemosensory-associated proteins, the odorant receptor co-receptor (Orco) and the transient receptor potential A (TRPA1) channel. While knockdown of Orco did not change the reaction of adult females to citronellal, TRPA1 silencing effectively eliminated the induced increase to movement, suggesting a chemoperceptory role in citronellal detection.

中文翻译:

TRPA1调节百日草中有害的气味反应。

百里香(Lygus hesperus)是北美西部许多重要经济作物的主要害虫。这些昆虫生活的许多方面的中心是化学信号,在宿主植物的选择,聚集和被动配偶保护中具有确定的作用。对这种昆虫的新型监测和控制方法的开发将取决于对这些线索如何被感知和处理以及它们对行为的影响的良好理解。为此,我们研究了异硫氰酸烯丙酯,肉桂醛和香茅醛,它们是对其他昆虫的有害驱避剂。我们发现阔叶猴避开了包含这三种化合物的区域,暴露导致若虫和成虫的运动速度和持续时间增加。这表明这些化合物可以作为驱避剂。为了更好地了解此反应的潜在生理学,使用dsRNA注射RNA干扰来抑制两种化学感觉相关蛋白的表达,即气味受体共受体(Orco)和瞬时受体电位A(TRPA1)通道。虽然敲低Orco并没有改变成年雌性对香茅醛的反应,但TRPA1沉默有效地消除了运动引起的增加,表明在香茅醛检测中化学感受器的作用。
更新日期:2020-03-20
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