This study investigated the influence of meat and bone meal (MBM), phytase, and antibiotics (AB) on the performance, intestinal pH, ileal digestibility, cecal microbiota, and tibial mineralization in Ross 308 broilers challenged with necrotic enteritis (NE). A total of 672-day-old male Ross 308 chicks were allocated to 8 treatments with 6 replicate pens, with 14 birds each. The study employed a 2 × 2 × 2 factorial arrangement of treatments: MBM (no or yes), AB (no or yes, zinc bacitracin + salinomycin), and phytase level (500 or 1,500 FTU/kg; both using 500 matrix recommendations). Diets were based on wheat-soybean meal-canola meal. All birds were challenged with Eimeria spp on day 9 and Clostridium perfringens (C. perfringens) strain EHE-NE18 on day 14 and day 15. On day 21 (postchallenge), birds fed MBM had reduced weight gain (WG; P < 0.05) relative to without MBM. A 2-way phytase × AB interaction for WG on day 14 (P < 0.001) and day 21 (P < 0.001) and feed conversion ratio on day 21 (P < 0.001) and day 42 (P < 0.01) indicated positive effects of high phytase on bird performance in the presence of AB. On day 42, a 3-way MBM × phytase × AB interaction (P < 0.01) was observed for WG, showing high phytase increased WG with AB, relative to the birds without AB in the presence of MBM. A 2-way MBM × phytase interaction (P < 0.01) was observed for apparent ileal digestibility of Ca and P on day 16, whereby there was a notable reduction in Ca and P digestibility in birds fed MBM-free diets and a low phytase level, but with the high phytase level, Ca and P digestibility was not influenced by MBM. In conclusion, in NE challenged birds, high phytase has a beneficial effect on leg health and mineral utilization to the extent that it can replace MBM and has beneficial effects on bird performance in the presence of AB.

中文翻译：

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肉和骨粉，肌醇六磷酸酶和抗生素对受到亚临床坏死性肠炎攻击的肉鸡的影响：1.生长性能，肠道pH，表观回肠消化率，盲肠菌群和胫骨矿化。

这项研究调查了肉和骨粉（MBM），植酸酶和抗生素（AB）对Ross 308肉鸡在坏死性肠炎（NE）攻击下的性能，肠道pH，回肠消化率，盲肠菌群和胫骨矿化的影响。将总共​​672天的雄性Ross 308小鸡分配到8种处理中，每组有6只重复的钢笔。该研究采用2×2×2阶乘安排的处理方法：MBM（否或是），AB（否或是，杆菌肽锌+盐霉素）和植酸酶水平（500或1,500 FTU / kg；两者均使用500种基质推荐） 。饮食以小麦-大豆粉-低芥酸菜粕为基础。在第9天，所有鸟类都受到艾美叶球菌的侵袭，在第14天和第15天用产气荚膜梭状芽孢杆菌（C. perfringens）菌株EHE-NE18攻击。在攻击后第21天，用MBM饲喂的家禽体重减轻（WG; P <0）。05）相对于没有MBM。第14天（P <0.001）和第21天（P <0.001）的WG的双向植酸酶×AB相互作用以及第21天（P <0.001）和第42天（P <0.01）的饲料转化率表明植酸酶在AB存在下对禽类生长的影响 在第42天，观察到WG的3向MBM×植酸酶×AB相互作用（P <0.01），表明与存在MBM的无AB禽相比，具有AB的高植酸酶增加了WG。在第16天，观察到Ca和P的回肠消化率具有2种MBM×植酸酶相互作用（P <0.01），因此，饲喂无MBM饮食和低植酸酶水平的家禽的Ca和P消化率显着降低，但是由于植酸酶水平较高，Ca和P的消化率不受MBM的影响。总之，在东北挑战鸟类中，