The antibacterial properties of egg yolk antibodies have been known for many years. Enhanced antibiotic resistance has resulted in increased need for using these antibodies as an alternative. In the present study, generation, capsulation, and inhibition growth properties of IgY directed against Salmonella enterica subsp. enterica serovar Infantis (SI) were evaluated. White Leghorn layer hens were immunized using whole cell of inactivated SI. Salmonella Infantis-specific antibody activities in sera and egg yolk were determined by ELISA. A total of 480 one-day-old male "Cobb 500" chicks were randomly divided into 8 groups, with 6 replications of 10 birds kept for 21 D. All birds from 7 challenged groups were orally inoculated with 1 mL of SI suspension (1 × 107 CFU/mL) at 3 and 4 D of age. Two groups were dietary supplemented with 5 g/kg immune powdered yolk or nonimmune powdered yolk. One group was dietary supplemented with 12.8 g/kg capsulated immune yolk (CIY). Two groups were given 8.3 mL/L of immune water-soluble yolk or nonimmune water-soluble yolk fraction in drinking water. In the antibiotic group, 1 mL/L Enrofloxacin 10% was added to drinking water. All supplements except for the antibiotic (on Day 4 for 10 D) were added on day one and continued during the experiment. Negative and positive control groups received no supplements. During the experiment, among the challenged groups, the minimum SI cecal colonization and the lowest isolation of SI from the liver (P < 0.01) was observed in the antibiotic group. Following antibiotic group, in the group receiving CIY, colonization of bacteria in ceca and liver was significantly reduced during the second and third weeks of the experiment (P < 0.01). According to the results, capsulated specific IgY has a beneficial effect in reducing the colonization of Salmonella under the conditions of this study in comparison with other forms of IgY antibody.

中文翻译：

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胶囊化和非胶囊化蛋黄特异性抗体对减少肠道沙门氏菌定殖的作用。受婴儿肠炎感染的肉鸡。


蛋黄抗体的抗菌特性多年来已为人所知。抗生素耐药性的增强导致对使用这些抗体作为替代品的需求增加。在本研究中，针对肠沙门氏菌亚种的 IgY 的产生、包膜和抑制生长特性。对婴儿肠血清型（SI）进行了评估。使用灭活SI的全细胞对白来航蛋鸡进行免疫。通过 ELISA 测定血清和蛋黄中婴儿沙门氏菌特异性抗体活性。总共 480 只 1 日龄雄性“Cobb 500”雏鸡随机分为 8 组，每组 10 只，共 6 个重复，保存 21 天。7 个攻毒组的所有鸡均口服接种 1 mL SI 悬浮液（1 × 107 CFU/mL) 在 3 日龄和 4 日龄时。两组在膳食中添加 5 g/kg 免疫蛋黄粉或非免疫蛋黄粉。一组在膳食中添加 12.8 g/kg 的胶囊免疫卵黄 (CIY)。两组均在饮用水中给予8.3 mL/L的免疫水溶性蛋黄或非免疫水溶性蛋黄部分。抗生素组在饮用水中添加1 mL/L 10%恩诺沙星。除抗生素（第 4 天，共 10 天）外的所有补充剂均在第一天添加，并在实验期间继续进行。阴性和阳性对照组不接受任何补充剂。实验期间，在攻击组中，抗生素组观察到 SI 盲肠定植最少，SI 与肝脏分离最低 (P < 0.01)。继抗生素组之后，在接受 CIY 的组中，在实验的第二周和第三周期间，盲肠和肝脏中的细菌定植显着减少（P< 0.01）。 根据结果​​，与其他形式的 IgY 抗体相比，在本研究条件下，封装的特异性 IgY 对减少沙门氏菌定植具有有益作用。