Goose parvovirus (GPV) leads to a huge loss in the poultry industry, and early diagnosis is required to prevent the disease from spreading. At present, there are a variety of detection methods for GPV infection, and the ELISA method has the advantages of simple and rapid operation. However, most ELISA methods for detecting GPV can only detect the antibody level of the sample, but cannot distinguish between the GPV infection and vaccine immunization antibodies. Therefore, this study has a wider application value by establishing the detection method based on the structure and non-structural protein of the virus. The GPV non-structural (NS1) and structure (VP3) fusion proteins were used as coating antigens to establish 2 indirect ELISA methods, and the detection conditions were optimized. A series of experiments proved that the detection method has good specificity, sensitivity, and repeatability. The test results of 120 immune sera samples and 145 natural infection serum samples showed that the positive rates of immunized serum were 9.17% (NS1) and 88.33% (VP3), and the positive rates of natural infection were 88.97% (NS1) and 86.21% (VP3), which distinguish between the GPV infection and vaccine immunization antibodies. The establishment of 2 indirect ELISA methods using NS1 and VP3 proteins as inclusion antigens provides a new method for detecting GPV infection and inactivated immune antibodies, which lays a foundation for the serological diagnosis and epidemiological monitoring of GPV.

中文翻译：

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研究说明：ELISA的发展以区分鹅细小病毒感染和疫苗免疫抗体。

鹅细小病毒（GPV）导致家禽业的巨大损失，因此需要早期诊断以防止疾病传播。目前，存在针对GPV感染的多种检测方法，而ELISA法具有操作简便，快速的优点。但是，大多数用于检测GPV的ELISA方法只能检测样品的抗体水平，而不能区分GPV感染抗体和疫苗免疫抗体。因此，通过建立基于病毒的结构和非结构蛋白的检测方法，本研究具有广泛的应用价值。以GPV非结构（NS1）和结构（VP3）融合蛋白作为包被抗原，建立2种间接ELISA方法，并优化了检测条件。一系列实验证明，该检测方法具有良好的特异性，敏感性和可重复性。对120份免疫血清和145份自然感染血清的检测结果表明，免疫血清阳性率为9.17％（NS1）和88.33％（VP3），自然感染阳性率为88.97％（NS1）和86.21。 ％（VP3），用于区分GPV感染和疫苗免疫抗体。建立以NS1和VP3蛋白为包涵体抗原的两种间接ELISA方法，为检测GPV感染和免疫抗体失活提供了一种新方法，为GPV的血清学诊断和流行病学监测奠定了基础。对120份免疫血清和145份自然感染血清的检测结果表明，免疫血清阳性率为9.17％（NS1）和88.33％（VP3），自然感染阳性率为88.97％（NS1）和86.21。 ％（VP3），用于区分GPV感染和疫苗免疫抗体。建立以NS1和VP3蛋白为包涵体抗原的两种间接ELISA方法，为检测GPV感染和免疫抗体失活提供了一种新方法，为GPV的血清学诊断和流行病学监测奠定了基础。对120份免疫血清和145份自然感染血清的检测结果表明，免疫血清阳性率为9.17％（NS1）和88.33％（VP3），自然感染阳性率为88.97％（NS1）和86.21。 ％（VP3），用于区分GPV感染和疫苗免疫抗体。建立以NS1和VP3蛋白为包涵体抗原的两种间接ELISA方法，为检测GPV感染和免疫抗体失活提供了一种新方法，为GPV的血清学诊断和流行病学监测奠定了基础。