Biomaterial development for clinical applications is currently on the rise. This necessitates adequate in vitro testing, where the structure and composition of biomaterials must be specifically tailored to withstand in situ repair and regeneration responses for a successful clinical outcome. The chorioallantoic membrane of chicken embryos has been previously used to study angiogenesis, a prerequisite for most tissue repair and regeneration. In this study, we report an optimised ex ovo method using a glass-cling film set-up that yields increased embryo survival rates and has an improved protocol for harvesting biomaterials. Furthermore, we used this method to examine the intrinsic angiogenic capacity of a variety of biomaterials categorised as natural, synthetic, natural/synthetic and natural/natural composites with varying porosities. We detected significant differences in biomaterials' angiogenesis with natural polymers and polymers with a high overall porosity showing a greater vascularisation compared to synthetic polymers. Therefore, our proposed ex ovo chorioallantoic membrane method can be effectively used to pre-screen biomaterials intended for clinical application.

中文翻译：

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在优化的卵外绒毛尿囊膜模型中预筛选生物材料的内在血管生成能力。


用于临床应用的生物材料开发目前正在兴起。这就需要进行充分的体外测试，其中生物材料的结构和成分必须经过专门定制，以承受原位修复和再生反应，以获得成功的临床结果。鸡胚胎的绒毛尿囊膜以前曾被用来研究血管生成，这是大多数组织修复和再生的先决条件。在这项研究中，我们报告了一种使用玻璃保鲜膜装置的优化离卵方法，该方法可提高胚胎存活率，并改进了收获生物材料的方案。此外，我们使用这种方法来检查各种生物材料的内在血管生成能力，这些生物材料被分类为具有不同孔隙率的天然、合成、天然/合成和天然/天然复合材料。我们发现天然聚合物和具有高总体孔隙率的聚合物在生物材料的血管生成方面存在显着差异，与合成聚合物相比，显示出更大的血管形成。因此，我们提出的卵绒毛尿囊膜方法可以有效地用于预筛选用于临床应用的生物材料。