Discovered in the 1980s, small regulatory RNAs (sRNAs) are now considered key actors in virtually all aspects of bacterial physiology and virulence. Together with transcriptional and translational regulatory proteins, they integrate and often are hubs of complex regulatory networks, responsible for bacterial response/adaptation to various perceived stimuli. The recent development of powerful RNA sequencing technologies has facilitated the identification and characterization of sRNAs (length, structure and expression conditions) and their RNA targets in several bacteria. Nevertheless, it could be very difficult for non-experts to understand the advantages and drawbacks related to each offered option and, consequently, to make an informed choice. Therefore, the main goal of this review is to provide a guide to navigate through the twists and turns of high-throughput RNA sequencing technologies, with a specific focus on those applied to the study of sRNAs. This article is part of a Special Issue entitled: RNA and gene control in bacteria edited by Dr. M. Guillier and F. Repoila.

中文翻译：

---

在RNA测序技术的曲折中导航：应用于细菌调控RNA。

小调节RNA（sRNA）于1980年代发现，如今已被视为细菌生理和毒力几乎所有方面的关键角色。它们与转录和翻译调节蛋白一起整合，通常是复杂调节网络的枢纽，负责细菌对各种感知刺激的应答/适应。强大的RNA测序技术的最新发展促进了几种细菌中sRNA（长度，结构和表达条件）及其RNA靶标的鉴定和表征。然而，对于非专家而言，要理解与每个提供的选项有关的优缺点并因此做出明智的选择可能非常困难。因此，这篇综述的主要目的是为高通量RNA测序技术的曲折提供指导，特别是针对那些用于sRNA研究的技术。本文是M. Guillier博士和F. Repoila编辑的《细菌中的RNA和基因控制》特刊的一部分。