Studying substrate consumption in nutrient-rich conditions is challenging because often the growth medium includes undefined components like yeast extract or peptone. For clear and consistent results, it is necessary to use defined medium, where substrate utilization can be followed. In the present work, Escherichia coli BW25113 batch growth in a medium supplemented with 20 proteinogenic amino acids and glucose was studied. Focus was on the quantitative differences in substrate consumption and proteome composition between minimal and nutrient-rich medium. In the latter, 72% of carbon used for biomass growth came from amino acids and 28% from glucose. Serine was identified as the most consumed substrate with 41% of total carbon consumption. Proteome comparison between nutrient-rich and minimal medium revealed changes in TCA cycle and acetate producing enzymes that together with extracellular metabolite data pointed to serine being consumed mainly for energy generation purposes. Serine removal from the growth medium decreased specific growth rate by 22%. In addition, proteome comparison between media revealed a large shift in amino acid synthesis and translation related proteins. Overall, this work describes in quantitative terms the batch growth carbon uptake profile and proteome allocation of E. coli BW25113 in minimal and nutrient-rich medium.

在营养丰富的条件下研究底物消耗具有挑战性，因为生长培养基通常包含不确定的成分，例如酵母提取物或蛋白ept。为了获得清晰一致的结果，有必要使用确定的介质，可以跟踪底物利用率。在目前的工作中，大肠杆菌研究了BW25113在补充20种蛋白原氨基酸和葡萄糖的培养基中的批量生长。重点在于基本培养基和营养丰富的培养基之间底物消耗和蛋白质组组成的定量差异。在后者中，用于生物量生长的碳有72％来自氨基酸，而28％来自葡萄糖。丝氨酸被确定为最消耗的底物，占总碳消耗的41％。富含营养的培养基和基本培养基之间的蛋白质组比较显示，TCA周期和产生乙酸盐的酶发生了变化，再加上细胞外代谢物数据表明，丝氨酸主要用于产生能量。从生长培养基中去除丝氨酸使比生长速率降低了22％。此外，蛋白质之间的蛋白质组比较显示氨基酸合成和翻译相关蛋白发生了很大变化。总的来说，这项工作以定量的方式描述了批次生长碳的吸收概况和蛋白质组的分配。在基本且营养丰富的培养基中的大肠杆菌BW25113。