Toxoplasma gondii is an intracellular parasite that infects a broad range of animal species and humans. As the main surface antigen of the tachyzoite, SAG1 is involved in the process of recognition, adhesion and invasion of host cells. The aim of the current systematic review study is to clarify the latest status of studies in the literature regarding SAG1-associated recombinant proteins or SAG1-associated recombinant DNAs as potential vaccines against toxoplasmosis. Data were systematically collected from six databases including PubMed, Science Direct, Web of Science, Google Scholar, EBSCO and Scopus, up to 1st of January 2019. A total of 87 articles were eligible for inclusion criteria in the current systematic review. The most common antigens used for experimental cocktail vaccines together with SAG1 were ROP2 and SAG2. In addition, the most parasite strains used were RH and ME49. Freund's adjuvant and cholera toxin have been predominantly utilized. Furthermore, regarding the animal models, route and dose of vaccination, challenge methods, measurement of immune responses and cyst burden have been discussed in the text. Most of these experimental vaccines induce immune responses and have a high degree of protection against parasite infections, increase survival rates and duration and reduce cyst burdens. The data demonstrated that SAG1 antigen has a high potential for use as a vaccine and provided a promising approach for protecting humans and animals against toxoplasmosis.

中文翻译：

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弓形虫表面抗原1（SAG1）作为开发抗弓形虫病疫苗的潜在候选者：一项系统综述。

弓形虫是一种感染多种动物和人类的细胞内寄生虫。作为速殖子的主要表面抗原，SAG1参与宿主细胞的识别，粘附和侵袭过程。当前系统综述研究的目的是阐明有关SAG1相关重组蛋白或SAG1相关重组DNA作为弓形虫病潜在疫苗的文献研究的最新状态。截至2019年1月1日，已从包括PubMed，Science Direct，Web of Science，Google Scholar，EBSCO和Scopus在内的六个数据库系统地收集了数据。共有87篇文章符合当前系统评价的纳入标准。与SAG1一起用于实验性鸡尾酒疫苗的最常见抗原是ROP2和SAG2。此外，使用最多的寄生虫菌株是RH和ME49。主要使用了弗氏佐剂和霍乱毒素。此外，关于动物模型，疫苗接种途径和剂量，攻击方法，免疫应答和囊肿负担的测量方法，已在本文中进行了讨论。这些实验性疫苗中的大多数会诱导免疫反应，并具有高度的保护力，可抵御寄生虫感染，提高存活率和持续时间并减轻囊肿负担。数据表明，SAG1抗原具有用作疫苗的巨大潜力，并为保护人类和动物免受弓形虫病提供了一种有前途的方法。免疫反应和囊肿负担的测量方法已在本文中讨论。这些实验性疫苗中的大多数会诱导免疫反应，并具有高度的保护力，可抵御寄生虫感染，提高存活率和持续时间并减轻囊肿负担。数据表明，SAG1抗原具有用作疫苗的巨大潜力，并为保护人类和动物免受弓形虫病提供了有希望的方法。免疫反应和囊肿负担的测量方法已在本文中讨论。这些实验性疫苗中的大多数会诱导免疫反应，并具有高度的保护力，可抵御寄生虫感染，提高存活率和持续时间并减轻囊肿负担。数据表明，SAG1抗原具有用作疫苗的巨大潜力，并为保护人类和动物免受弓形虫病提供了有希望的方法。