BACKGROUND For the diagnosis and treatment of adult T-cell leukemia/lymphoma (ATLL) caused by human T-lymphotropic virus type 1 (HTLV-1) are required therapeutic modalities urgently. Non-human primate models for ATLL would provide a valuable information for clinical studies. We did a pilot study to establish an ATLL non-human primate model using common marmosets (Callithrix jacchus). METHODS We inoculated HTLV-1-producing MT-2 cells into 9-month-old marmosets, either intraperitoneally or intravenously. We next administrated MT-2 cells into 13-month-old marmosets under cyclosporine A (CsA) treatment to promote infection. HTLV-1 infection was determined by measuring HTLV-1 antibody titer in the common marmosets. RESULTS The HTLV-1 antibody titer increased in the intraperitoneally inoculated marmoset with or without CsA treatment, and it kept over five 5 years though proviral copy number (proviral load, PVL) remained low throughout the study. CONCLUSION We obtained HTLV-1 asymptomatic carriers of common marmosets by inoculating MT-2 cells.

中文翻译：

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建立使用普通mar猴（Callithrix jacchus）建立1型人T细胞白血病病毒（HTLV-1）携带者模型的初步研究。

背景技术对于由人1型T淋巴细胞病毒（HTLV-1）引起的成人T细胞白血病/淋巴瘤（ATLL）的诊断和治疗是迫切需要的治疗方式。ATLL的非人类灵长类动物模型将为临床研究提供有价值的信息。我们进行了一项试点研究，以使用普通mar猴（Callithrix jacchus）建立ATLL非人类灵长类动物模型。方法我们腹膜内或静脉内将产生HTLV-1的MT-2细胞接种到9个月大的mar猴中。接下来，我们在环孢菌素A（CsA）处理下将MT-2细胞施用到13个月大的mos猴中，以促进感染。通过测量普通mar猴中的HTLV-1抗体滴度确定HTLV-1感染。结果在接受或不接受CsA处理的腹腔接种mar猴中，HTLV-1抗体滴度增加，并且在整个研究中，尽管前病毒拷贝数（实验量，PVL）仍然很低，但仍保持了5到5年。结论我们通过接种MT-2细胞获得了普通mar猴的HTLV-1无症状携带者。