当前位置：
X-MOL 学术
›
Mol. Plant Pathol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
The RpoN2-PilRX regulatory system governs type IV pilus gene transcription and is required for bacterial motility and virulence in Xanthomonas oryzae pv. oryzae.
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-02-29 , DOI: 10.1111/mpp.12920 Chao Yu 1 , Doan-Phuong Nguyen 1 , Zhaoyu Ren 1 , Jianan Liu 1 , Fenghuan Yang 1 , Fang Tian 1 , Susu Fan 2 , Huamin Chen 1
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-02-29 , DOI: 10.1111/mpp.12920 Chao Yu 1 , Doan-Phuong Nguyen 1 , Zhaoyu Ren 1 , Jianan Liu 1 , Fenghuan Yang 1 , Fang Tian 1 , Susu Fan 2 , Huamin Chen 1
Affiliation
|
The type IV pilus (T4P), a special class of bacterial surface filament, plays crucial roles in surface adhesion, motility, biofilm formation, and virulence in pathogenic bacteria. However, the regulatory mechanism of T4P and its relationship to bacterial virulence are still little understood in Xanthomonas oryzae pv. oryzae (Xoo), the causal pathogen of bacterial blight of rice. Our previous studies showed that the σ54 factor RpoN2 regulated bacterial virulence on rice in a flagellum‐independent manner in Xoo. In this study, both yeast two‐hybrid and pull‐down assays revealed that RpoN2 directly and specifically interacted with PilRX, a homolog of the response regulator PilR of the two‐component system PilS‐PilR in the pilus gene cluster. Genomic sequence and reverse transcription PCR (RT‐PCR) analysis showed 13 regulons containing 25 genes encoding T4P structural components and putative regulators. A consensus RpoN2‐binding sequence GGN10GC was identified in the promoter sequences of most T4P gene transcriptional units. Electrophoretic mobility shift assays confirmed the direct binding of RpoN2 to the promoter of the major pilin gene pilAX, the inner membrane platform protein gene pilCX, and pilRX. Promoter activity and quantitative RT‐PCR assays demonstrated direct and indirect transcriptional regulation by RpoN2 of the T4P genes. In addition, individual deletions of pilAX, pilCX, and pilRX resulted in significantly reduced twitching and swimming motility, biofilm formation, and virulence in rice. Taken together, the findings from the current study suggest that the RpoN2‐PilRX regulatory system controls bacterial motility and virulence by regulating T4P gene transcription in Xoo.
中文翻译：
RpoN2-PilRX调节系统控制IV型菌毛基因转录，是米氏黄单胞菌pv中细菌活力和致病力所必需的。水稻
IV型菌毛（T4P）是一类特殊的细菌表面细丝，在致病细菌的表面粘附，运动性，生物膜形成和毒力中起关键作用。然而，在稻草黄单胞菌PV中，对T4P的调节机制及其与细菌毒力的关系仍然知之甚少。米曲霉（白叶枯病），水稻细菌性枯萎病的致病病原体。我们以往的研究表明，σ 54RooN2因子以X鞭毛不依赖鞭毛的方式调节水稻的细菌毒力。在这项研究中，酵母双杂交和下拉分析均显示RpoN2与PilRX直接特异性相互作用，PilRX是菌毛基因簇中两组分系统PilS-PilR的响应调节因子PilR的同源物。基因组序列和逆转录PCR（RT-PCR）分析显示13个调控子包含25个编码T4P结构成分和推定调节子的基因。在大多数T4P基因转录单位的启动子序列中鉴定出一个共有的RpoN2结合序列GGN 10 GC。电泳迁移率变动分析证实RpoN2与主要pilin基因pilAX（内膜平台蛋白基因）的启动子直接结合pilCX和pilRX。启动子活性和定量RT-PCR分析证明了RpoN2对T4P基因的直接和间接转录调控。此外，单独删除pilAX，pilCX和pilRX会导致水稻的抽搐和游泳运动，生物膜形成和毒力显着降低。综上所述，当前研究的结果表明，RpoN2-PilRX调节系统通过调节Xoo中的T4P基因转录来控制细菌的运动性和毒力。
更新日期：2020-02-29
中文翻译：
RpoN2-PilRX调节系统控制IV型菌毛基因转录，是米氏黄单胞菌pv中细菌活力和致病力所必需的。水稻
IV型菌毛（T4P）是一类特殊的细菌表面细丝，在致病细菌的表面粘附，运动性，生物膜形成和毒力中起关键作用。然而，在稻草黄单胞菌PV中，对T4P的调节机制及其与细菌毒力的关系仍然知之甚少。米曲霉（白叶枯病），水稻细菌性枯萎病的致病病原体。我们以往的研究表明，σ 54RooN2因子以X鞭毛不依赖鞭毛的方式调节水稻的细菌毒力。在这项研究中，酵母双杂交和下拉分析均显示RpoN2与PilRX直接特异性相互作用，PilRX是菌毛基因簇中两组分系统PilS-PilR的响应调节因子PilR的同源物。基因组序列和逆转录PCR（RT-PCR）分析显示13个调控子包含25个编码T4P结构成分和推定调节子的基因。在大多数T4P基因转录单位的启动子序列中鉴定出一个共有的RpoN2结合序列GGN 10 GC。电泳迁移率变动分析证实RpoN2与主要pilin基因pilAX（内膜平台蛋白基因）的启动子直接结合pilCX和pilRX。启动子活性和定量RT-PCR分析证明了RpoN2对T4P基因的直接和间接转录调控。此外，单独删除pilAX，pilCX和pilRX会导致水稻的抽搐和游泳运动，生物膜形成和毒力显着降低。综上所述，当前研究的结果表明，RpoN2-PilRX调节系统通过调节Xoo中的T4P基因转录来控制细菌的运动性和毒力。
京公网安备 11010802027423号